Chapter 18, Problem 2UTM

4

Does it show the level of proteins? What about the amount? Levels of protein activation? How can you tell? Does the thickness tell you anything? What about the number of the lines? And the other questions

KD 200- 116- 66- Vec ATF6 (670) ATF6 (402) ATF6 (373) ATF6 (366) I I 45- 1 2 3 4 5 ATFG (360) (e/c) 9V ATFG (402) g ant- ATF anti-KDEL DAPI b

Western blot results: what information can you get? Presence of proteins of your interest Levels of protein expression Levels of protein activation (must use activation state-specific antibody) Decreased function of the ATM kinase in aging mice. A C57BL/6 female 6 month Con IR 20 month C57BL/6 male 6 month 28 month Con IR Con IR Con IR p-ATM (S1981) ATM P-p53 (ser18) Actin

Does it show the level of proteins? What about the amount? Levels of protein activation? How can you tell? Does the thickness tell you anything? What about the number of the lines?

WB: Protein of interest visualized by fluorescent Protein A Protein B

Question #4: Assume you are able to use CRISPR to create an allele that will convert a cross-pollinated, sexually reproducing crop plant into an obligate apomict. Your edited obligate apomict plants retains all the CRISPR "machinery" necessary to convert the "sexually reproducing" allele to the "obligate apomict" allele. You plant 100 hectares of your edited obligate apomicts in order to increase seed for sale the following year. Neighboring farms and seed producers are growing many different un-edited sexually reproducing varieties of the crop. If your neighbors plant seed harvested from their crops that was pollinated by your crop, should they expect these seeds to generate apomictic or non-apomictic plants? Type your answer here:

calculate the questions showing the solution including variables,unit and equations all the questiosn below using the data.show solving and answer a) B1,   b) B2,   c) hybrid rate constant (1) d) hybrid rate constant (2) e) t1/2,dist t1/2,absorb f) t1/2,elim   k) apparent central compartment volume (V1,app) p) total AUC (using short cut method) apparent volume of distribution based on AUC (VAUC,app)  apparent clearance (CLapp)   absolute bioavailabilty of oral route ( AUCiv =116ml)

Please help me to draw this by hand. In as much detail as possible, hand draw a schematic diagram of the hypothalamic-pituitary-gonad (HPG) axis in the human female. Be sure to include all the relevant structures and hormones. You must define all abbreviations the first time you use them. Please include (and explain) the feedback loops.