a. To determine: The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein). Introduction: The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

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Answer 1

Question

Chapter 18, Problem 16P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

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19. On the diagram below a. Label the three pictures as: DNA; polypeptide; or RNA. b. Label the arrows as: translation or transcription/RNA processing. c. Add the following details to the diagram. Promoter region TATA box Transcription start site Transcription terminator Intron (A,B,C,D) Exons (1,2,3,4,5) Splice sites 5' cap 5' UTR (untranslated region) 3' poly A tail 3' UTR (untranslated region) Translational start (AUG) Translational stop (UGA, UAG, or UAA) N and C ends of polypeptide 0000

Match the letter labels in the figure below to the terms. Some letter labels are not used. MNNNNNNIN M C B A M D F E H K G 8

The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions. 1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction? 2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe: a) What molecule do you recommend for the trial. Why? b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for? 3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale. 4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale. Mutation 1 Mutation 2

Answer 2

Question

Chapter 18, Problem 16P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

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Answer 3

Question

Chapter 18, Problem 16P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

Expert Solution & Answer

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Answer 4

Question

Chapter 18, Problem 16P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

Expert Solution & Answer

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Answer 5

Chapter 18, Problem 16P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

Answer 6

Chapter 18, Problem 16P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Answer 7

Chapter 18, Problem 16P

Summary Introduction

a.

To determine:

The place in the genome where a gene trapping construct can be integrated efficiently for the expression of GFP (Green fluorescent protein).

Introduction:

The enhancer trapping technique was developed by researchers for the very first time in Drosophila. This technique is utilized for finding enhancers in the genome of the organism.

Answer 8

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Answer 9

Summary Introduction

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

Answer 10

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Answer 11

Summary Introduction

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

Answer 12

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

Answer 13

Summary Introduction

d.

To determine:

The method among gene trapping and enhancer trapping which can be used to produce a mutant allele of a particular gene adjacent to the integration site.

Introduction:

The constructs of DNA (Deoxyribose nucleic acid) are incorporated into the ES cells of the mouse. Majority of recombination events occurs at the random sites in such cases, and also the homologous recombination is usually rare.

Answer 14

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Answer 15

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Answer 16

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Answer 17

Ch. 18 - Match each of the terms in the left column to the...Ch. 18 - Mice are usually gray, but a mouse geneticist has...Ch. 18 - Sometimes, genes transferred into the mouse genome...Ch. 18 - In mice, a group of so-called Hox genes encode...Ch. 18 - The fly eyes shown in Fig. 18.7 are malformed...Ch. 18 - This problem concerns a technique called enhancer...Ch. 18 - Fish and other organisms that live in the Arctic...Ch. 18 - a. Describe two ways you could potentially make a...Ch. 18 - Figure 18.6 shows a picture of Glofish ,...Ch. 18 - Some people are concerned about the possible...

Solution Summary: The author describes gene trapping as a high-throughput approach that is utilized for the insertional type of mutations across the genome of mammals.

Concept explainers

Videos

b.

To determine:

Whether the genome of all mice should be integrated in a way which is described in the previous part.

Introduction:

A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

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Chapter 18 Solutions

Solution Summary: The author describes gene trapping as a high-throughput approach that is utilized for the insertional type of mutations across the genome of mammals.

Concept explainers

Videos

b. To determine: Whether the genome of all mice should be integrated in a way which is described in the previous part. Introduction: A similar type of technique called gene trapping is used in the case of mice. In this technique a particular exon codes for a reporter type of protein such as GFP.

c. To determine: The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques. Introduction: The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.

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Chapter 18 Solutions

c.

To determine:

The way by which obtained information about genes varies in enhancer trapping and gene trapping techniques.

Introduction:

The GFP gene is incorporated or integrated randomly at different locations in the chromosomes of ES cells.