What is Life? A Guide to Biology with Physiology
3rd Edition
ISBN: 9781464157745
Author: Jay Phelan
Publisher: W. H. Freeman
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Chapter 17, Problem 6SA
Summary Introduction
To analyze:
Why are most leaves thin despite coming in various shapes and sizes?
Introduction:
Leaves of different plants have different shapes and sizes and despite this fact, most of the leaves are thin. Leaves are usually a three layered structure that is responsible for capturing energy and transport of water and nutrients.
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Overview of Transformation Protocol
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Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
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Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 17 Solutions
What is Life? A Guide to Biology with Physiology
Ch. 17 - Prob. 1SACh. 17 - Prob. 2SACh. 17 - Prob. 3SACh. 17 - Prob. 4SACh. 17 - Prob. 5SACh. 17 - Prob. 6SACh. 17 - Prob. 7SACh. 17 - Prob. 8SACh. 17 - Prob. 9SACh. 17 - Prob. 10SA
Ch. 17 - Prob. 11SACh. 17 - Prob. 12SACh. 17 - Prob. 13SACh. 17 - Prob. 14SACh. 17 - Prob. 1MCCh. 17 - Prob. 2MCCh. 17 - Prob. 3MCCh. 17 - Prob. 4MCCh. 17 - Prob. 5MCCh. 17 - Prob. 6MCCh. 17 - Prob. 7MCCh. 17 - Prob. 8MCCh. 17 - Prob. 9MCCh. 17 - Prob. 10MCCh. 17 - Prob. 11MCCh. 17 - Prob. 12MCCh. 17 - Prob. 13MCCh. 17 - Prob. 14MC
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