Chapter 17, Problem 6PDQ

Summary Introduction

To review:

The palindromic recognition sequence in $\text{5'-CAGTATGGATCCCAT-3'}$ for a restriction enzyme. Also, show the double-stranded DNA (deoxyribonucleic acid) sequence of the palindrome and the enzymes that would cut at the palindromic sequence.

Introduction:

The deliberate change (modifications) in the genetic material of an organism by altering the nucleic acid is called genetic engineering, which is generally accomplished by RDT (recombinant DNA or deoxyribonucleic acid). The fragment of DNA to be cloned in the vector molecule is called DNA insert or foreign DNA that amplifies in a specific host. RDT involves many biological agent and tools for gene cloning, such as nucleases, DNA ligase, DNA insert, vector molecule, host cell, and kinasesor phosphatases.