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Concept explainers
To review:
The reason for use of wide host range in RDT (recombinant DNA or deoxyribonucleic acid technology) for the transformation of recombinant DNA into a bacterial cell.
Introduction:
RDT involves gene cloning and genetic engineering process, in which manipulation of the genetic material of an organism is carried out. It is done by inserting the gene of interest in the vector, which carries it to a specific host to produce multiple new genetic combinations with the help of RDT. There are many tools that are used in the gene cloning experiment, such as restriction endonucleases, DNA ligases, foreign DNA or DNA insert, vector, and the host cell. Moreover, the host cell supports the amplification of the DNA fragment for gene expression at the
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Chapter 17 Solutions
Essentials of Genetics (9th Edition) - Standalone book
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- Describe how insulin binding to its receptor induces glucose uptake in healthy individuals. Please specifyeach step in the cascade and use a diagram to illustrate the answer.arrow_forwardThere is a patient with breast cancer, after staining the breast tissue with H&E, state the molecular subtype of the tumour extracted. Results of H&E staining are down belowarrow_forwardBiopharmaceutics and Pharmacokinetics:Two-Compartment Model Instant Absorption Questions Calculate these : a) B1, b) B2, c) hybrid rate constant (1) d) hybrid rate constant (2) e) t1/2,dist f) t1/2,elim g) k10 h) k12 i) k21 j) initial concentration (C0) k) central compartment volume (V1) l) steady-state volume (Vss) m) clearance (CL) AUC (0→10 min) using trapezoidal rule n) AUC (20→30 min) using trapezoidal rule o) AUCtail (AUC360→∞) p) total AUC (using short cut method) q) volume from AUC (VAUC)arrow_forward
- Use the Henderson-Hasselbalch equation for a propanoic acid solution (CH₂CH₂CO₂H, pK₁ = 4.874) to calculate the quotient [A-]/[HA] at three different pH values. pH = 4.479 [A-] [HA] [A-] pH = 4.874 [HA] = pH = 5.220 [A-] = [HA]arrow_forwardIn order to establish the expiration date of perishable food, growth curve data must be collected. Once the microbial load is so high that it poses a hazard to human health, the food item is no longer considered safe (expired). Generally a load of x50,000 bacteria/gram is considered unsafe. Your task is to determine the microbial growth curves for MicroYo, a new brand of yogurt. The growth is determined by sampling the yogurt and growing the bacterial isolates in broth culture which is then serially diluted by a total of x10,000 and inoculated onto standard petri plates of nutrient agar. The following colony counts are measured: Time (days) MicroYo colony count# 1 1 4 1 12 2 16 20 4 7 What day should you recommend expiring the yogurt (the last possible date before the microbial load is unsafe). 12 4 20 16arrow_forward9. Chicken combs in chickens is an example where you see interactions between genes. See potential genotypes and phenotypes below. Which genotype, when mated to a rose comb chicken, will produce progeny that are 50% walnut comb and 50% pea comb? walnut (RRPP) walnut (RrPP) pea (rrPP) walnut (RRPP) walnut (RrPp) pea (rrPp) rose rose single (RRPP) (Rrpp) (rrpp)arrow_forward
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- Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxConcepts of BiologyBiologyISBN:9781938168116Author:Samantha Fowler, Rebecca Roush, James WisePublisher:OpenStax College
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