Chapter 17, Problem 5PDQ

Summary Introduction

To review:

The reason for use of wide host range in RDT (recombinant DNA or deoxyribonucleic acid technology) for the transformation of recombinant DNA into a bacterial cell.

Introduction:

RDT involves gene cloning and genetic engineering process, in which manipulation of the genetic material of an organism is carried out. It is done by inserting the gene of interest in the vector, which carries it to a specific host to produce multiple new genetic combinations with the help of RDT. There are many tools that are used in the gene cloning experiment, such as restriction endonucleases, DNA ligases, foreign DNA or DNA insert, vector, and the host cell. Moreover, the host cell supports the amplification of the DNA fragment for gene expression at the phenotypic level.