Chapter 17, Problem 4PDQ

Summary Introduction

To review:

The mechanism by which a gene that is modified by mRNA processing, can be cloned to produce insulin.

Introduction:

Recombinant protein produced by transferring the gene into the selected host cell with the help of cloning experiments and genetic engineering, thus leads to the generation of recombinant protein. Recombinant protein synthesis in a host cell is based on recombinant DNA technology (RDT).

Moreover, a recombinant protein is an important component of pharmaceuticals and thus leads to the production of recombinant protein at a large scale in the host cell (bacterial cell system, mammalian cell culture etc.) and a number of useful products can synthesize by this technique like human growth hormone, erythropoietin, and blood clotting factors, tissue plasminogen activator and insulin, which show therapeutic use for human welfare.