PRESCOTT'S MICROBIO W/PROCTORIO
11th Edition
ISBN: 9781264731060
Author: WILLEY
Publisher: MCG
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Chapter 17, Problem 4RC
Summary Introduction
To describe: The capacity of proteins to recognize and binds to DNA.
Introduction: Proteins are the
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a) Find a nitrogen base. What carbon is the base attached to?
b) What two nitrogenous bases are held together by 2 hydrogen bonds?
c)What two nitrogenous bases are held together by 3 hydrogen bonds?
You will be building a double-stranded DNA molecule, representing a short (20 base) sequence that could be found in a chromosome. It will have a 3' --> 5' strand and a 5' --> 3' strand.
Use two full 12-base-pair DNA models.
a) Upload a photo of a model once the first 6 nucleotides have been inserted to base pair with the two parent strands.
b)Upload a photo of DNA polymerase I and Ligase in "action" removing the RNA primer nucleotides and making new phosphodiester bonds.
c)Upload a photo of the final two DNA strands, after Termination, identifying the parent and daughter strands of each.
Write down the 12 base-pairs (ex A-T or G-C) contained in the two models you will us from top to bottom below
Model 1 bp:
Model 2 bp:
Chapter 17 Solutions
PRESCOTT'S MICROBIO W/PROCTORIO
Ch. 17.1 - Examine the uncut piece of DNA shown in the upper...Ch. 17.1 - Which of the above enzymes yield blunt ends? Which...Ch. 17.1 - Prob. 3MICh. 17.1 - What would you conclude if you obtained only blue...Ch. 17.1 - Why must introns be removed from eukaryotic DNA...Ch. 17.1 - Which plasmid is a shuttle vector? Why?Ch. 17.1 - In what ways does the BAC shown here differ from...Ch. 17.1 - Describe restriction enzymes, sticky ends, and...Ch. 17.1 - What is cDNA? Why is it necessary to generate cDNA...Ch. 17.1 - Prob. 3CC
Ch. 17.1 - Prob. 4CCCh. 17.1 - Prob. 5CCCh. 17.2 - Why, after three cycles, are the vast majority of...Ch. 17.2 - Briefly describe the polymerase chain reaction....Ch. 17.2 - Why is PCR used to detect infectious agents that...Ch. 17.2 - How would you use PCR to measure the concentration...Ch. 17.2 - Why is it possible to visualize a PCR product on...Ch. 17.2 - Prob. 5CCCh. 17.3 - Why are long fragments (e.g., 20,000 bp) of...Ch. 17.4 - What special considerations are necessary if one...Ch. 17.4 - Prob. 1CCCh. 17.4 - Prob. 2CCCh. 17.4 - Prob. 3CCCh. 17.4 - You are studying chemotaxis proteins in a newly...Ch. 17.5 - Prob. 1MICh. 17.5 - Prob. 1CCCh. 17.5 - Prob. 2CCCh. 17 - Which of the DNA molecules shown are recombinant?Ch. 17 - Prob. 1RCCh. 17 - Prob. 2RCCh. 17 - Prob. 3RCCh. 17 - Prob. 4RCCh. 17 - Prob. 5RCCh. 17 - Prob. 6RCCh. 17 - Prob. 1ALCh. 17 - Prob. 2ALCh. 17 - Suppose you transformed a plasmid vector carrying...Ch. 17 - You are interested in the activity and regulation...Ch. 17 - Prob. 5ALCh. 17 - Prob. 6ALCh. 17 - Prob. 7AL
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- a)What region of the DNA do general transcription factors and RNA polymerase bind to? b)Where, in a eukaryotic cell, can one find TFs? c)What are the TFs called that bind to Enhancer Sites? d)Describe the role of Activators and Repressors in development.arrow_forwarda) Describe how mRNA and tRNA interact. b)Translate the following mRNA codons: AUG GUU AAC CAG UGA c) What are transcription factors made of?arrow_forwarda) State the Central Dogma of Molecular Biology in your own words. b) What enzyme synthesizes mRNA? c) Describe mRNA splicing.arrow_forward
- Give me detailed Solution. Don't give Ai generated solutionarrow_forwarda) The lux operon is under positive control. Based on this information, does the luxR regulator sequence make a repressor protein or an activator protein? b) How will binding of this complex affect RNA polymerase? Remember this operon is under positive control. c) AHL is a signal molecule that V. fisheri makes to communicate with neighboring bacterial cells. This molecule can diffuse outside of the cell and into another bacterial cell in close proximity. This type of communication between bacterial cells is known as quorum sensing. If bacterial cell density is low how will this affect the lux operon? What will happen if the density is high?arrow_forward1) Given an mRNA with the following sequence, please translate the codons to a chain of amino acids. Use the codon chart below.5’AUG/CCU/GCU/UAC/CGG/GAG/UAA3’ “met-_________-_________-_________-_________-_________”STOP” 2) Assuming the original polypeptide chain below, match each type of point mutation with the polypeptide chain that results. Original polypeptide: Pro-Thr-Ser-Leu-Leu-His-Asn A. Missense B. Silent C. Nonsense D. Frameshift ______ Pro-Thr-Ser-STOP ______ Pro-Thr-Ser-Leu-Ile-His-Asn ______ Pro-Thr-Ser-Leu-Leu-His-Asn _______ Pro-Thr-His-Cys-Tyr-Thrarrow_forward
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