PRESCOTT'S MICROBIO W/PROCTORIO
11th Edition
ISBN: 9781264731060
Author: WILLEY
Publisher: MCG
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Chapter 17.2, Problem 3CC
How would you use PCR to measure the concentration of a specific gene’s mRNA?
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Chapter 17 Solutions
PRESCOTT'S MICROBIO W/PROCTORIO
Ch. 17.1 - Examine the uncut piece of DNA shown in the upper...Ch. 17.1 - Which of the above enzymes yield blunt ends? Which...Ch. 17.1 - Prob. 3MICh. 17.1 - What would you conclude if you obtained only blue...Ch. 17.1 - Why must introns be removed from eukaryotic DNA...Ch. 17.1 - Which plasmid is a shuttle vector? Why?Ch. 17.1 - In what ways does the BAC shown here differ from...Ch. 17.1 - Describe restriction enzymes, sticky ends, and...Ch. 17.1 - What is cDNA? Why is it necessary to generate cDNA...Ch. 17.1 - Prob. 3CC
Ch. 17.1 - Prob. 4CCCh. 17.1 - Prob. 5CCCh. 17.2 - Why, after three cycles, are the vast majority of...Ch. 17.2 - Briefly describe the polymerase chain reaction....Ch. 17.2 - Why is PCR used to detect infectious agents that...Ch. 17.2 - How would you use PCR to measure the concentration...Ch. 17.2 - Why is it possible to visualize a PCR product on...Ch. 17.2 - Prob. 5CCCh. 17.3 - Why are long fragments (e.g., 20,000 bp) of...Ch. 17.4 - What special considerations are necessary if one...Ch. 17.4 - Prob. 1CCCh. 17.4 - Prob. 2CCCh. 17.4 - Prob. 3CCCh. 17.4 - You are studying chemotaxis proteins in a newly...Ch. 17.5 - Prob. 1MICh. 17.5 - Prob. 1CCCh. 17.5 - Prob. 2CCCh. 17 - Which of the DNA molecules shown are recombinant?Ch. 17 - Prob. 1RCCh. 17 - Prob. 2RCCh. 17 - Prob. 3RCCh. 17 - Prob. 4RCCh. 17 - Prob. 5RCCh. 17 - Prob. 6RCCh. 17 - Prob. 1ALCh. 17 - Prob. 2ALCh. 17 - Suppose you transformed a plasmid vector carrying...Ch. 17 - You are interested in the activity and regulation...Ch. 17 - Prob. 5ALCh. 17 - Prob. 6ALCh. 17 - Prob. 7AL
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- What would be your experimental strategy and the lab materials required to clone the complementary human gene using a mammalian gene in conjunction with PCR?arrow_forwardYou are performing an experiment using CRISPR-cas9 to genetically modify the LacZ gene of a culture of E. coli. After you run the experiment, you decide to use gel electrophoresis to genotype the different bacterial cultures to determine if the gene editing was successful. How could your electrophoresis results confirm that the PCR was successful? And how could your electrophoresis results confirm that you successfully extracted genomic DNA from your bacterial samples?arrow_forwardWhat are the main differences between whole genome sequencing and whole exome sequencing?arrow_forward
- What are the advantages of Next Generation Sequencing?arrow_forwardDescribe the method of a PCR technique in which you can amplify fragments randomly? Briefly.arrow_forwardb) Describe how DNA is digested by different restriction enzymes c) Describe how gel electrophoresis is used to estimate the size of DNA fragments.arrow_forward
- What is the purpose of adding a primer to a PCR reaction? Is this primer made up of DNA or RNA nucleotides? Explain your reasoning.arrow_forwardHow did the private corporation Celera Genomics approach the sequencing of the human genome? What was the advantage of this approach?arrow_forwardIn PCR, the size of the product is based on A) the position of the primers. B) the amount of DNA you add to the reaction. (c) the number of cycles the process has gone through. D) the amount of enzyme you add to the reaction. the location of the machine.arrow_forward
- What information can be gathered by using DNA gel electrophoresis? Describe the steps in the process and explain how and why gel electrophoresis can be used to separate DNA fragments.arrow_forwardIn PCR amplification Why is it important to know the length of the sequence you amplify?arrow_forwardA double-stranded length of DNA is exposed to a restriction enzyme. The enzyme finds 3 recognition sites. How many fragments will be produced? a) 2 b) 3 c) 4 d) 5arrow_forward
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