Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
2nd Edition
ISBN: 9780134702339
Author: Erin C. Amerman
Publisher: PEARSON
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Textbook Question
Chapter 16.2, Problem 7QC
What are the tropic hormones of the anterior pituitary gland? What glands do they affect?
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 16 Solutions
Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
Ch. 16.1 - How do the endocrine and nervous systems differ in...Ch. 16.1 - Prob. 2QCCh. 16.1 - Prob. 3QCCh. 16.1 - What are the two major classes of hormones, and...Ch. 16.1 - 5. How do synergistic and antagonistic hormones...Ch. 16.1 - What is the half-life of a hormone, and how is it...Ch. 16.1 - 7. What are the three types of stimuli that...Ch. 16.1 - How is hormone secretion generally regulated?Ch. 16.2 - 1. How do the anterior pituitary and posterior...Ch. 16.2 - What is the hypothalamic-hypophyseal portal...
Ch. 16.2 - 3. What are the target tissues and effects of...Ch. 16.2 - What are the target tissues and effects of...Ch. 16.2 - Which gland produces ADH and oxytocin, and from...Ch. 16.2 - How does the hypothalamus control the secretion of...Ch. 16.2 - 7. What are the tropic hormones of the anterior...Ch. 16.2 - Describe the target tissues and effects of growth...Ch. 16.3 - 1. What are thyroid follicles and how are they...Ch. 16.3 - Prob. 2QCCh. 16.3 - What are the main functions of thyroid hormones?Ch. 16.3 - 4. How are thyroid hormones produced? How is this...Ch. 16.3 - 5. What homeostatic imbalances may accompany...Ch. 16.3 - What are the target tissues and effects of...Ch. 16.3 - Prob. 7QCCh. 16.4 - 1. What are the three zones of the adrenal...Ch. 16.4 - 2. What are the target tissues and effects of...Ch. 16.4 - 3. What are the target tissues and effects of...Ch. 16.4 - What two hormones are produced by the adrenal...Ch. 16.4 - What is the relationship between the adrenal...Ch. 16.5 - What are the main target tissues of glucagon? What...Ch. 16.5 - What are the main target tissues of insulin?Ch. 16.5 - What are the signs and symptoms of the two types...Ch. 16.5 - 4. How do glucagon and insulin work together to...Ch. 16.6 - Prob. 1QCCh. 16.6 - Prob. 2QCCh. 16.6 - Prob. 3QCCh. 16.6 - Prob. 4QCCh. 16.7 - Which hormones primarily control fluid...Ch. 16.7 - 2. What is the role of each of these hormones...Ch. 16.7 - Prob. 3QCCh. 16.7 - Prob. 4QCCh. 16.7 - Prob. 5QCCh. 16.7 - 6. What is the role of each hormone in the stress...Ch. 16 - Mark the following statements as true or false. If...Ch. 16 - Which of the following is not a potential effect...Ch. 16 - 3. Which of the following hormones is/are produced...Ch. 16 - How does ADH affect the amount of water in the...Ch. 16 - Prob. 5CYRCh. 16 - 6. List the target tissues and effects of the...Ch. 16 -
7. The thyroid gland consists of:
a. follicle...Ch. 16 - 8. Which of the following is not an effect of...Ch. 16 - 9. Mark the following statements as true or false....Ch. 16 - 10 Fill in the blanks: A rise in free and would...Ch. 16 - 11. Which of the following statements correctly...Ch. 16 -
12. Fill in the blanks: The outer part of the...Ch. 16 - Which of the following is not an effect of...Ch. 16 - 14. Cortisol is:
a. a potent inhibitor of the...Ch. 16 - 15. Describe the components of the...Ch. 16 - Which of the following hormones is not an integral...Ch. 16 - 17. Which of the following statements about the...Ch. 16 - Explain how insulin and glucagon are antagonists.Ch. 16 - Prob. 19CYRCh. 16 - Match the following hormones with their correct...Ch. 16 - Mark the following statements as true or false. If...Ch. 16 - Predict the effects of a pancreatic tumor that...Ch. 16 - Prob. 2CYUCh. 16 - 3. A patient has a brain tumor that necessitates...Ch. 16 - 1. Ms. Reczkiewicz has her thyroid gland removed...Ch. 16 - A new diet guru claims hypersecretion of cortisol...Ch. 16 - Lets say that the dietary supplement in question 2...Ch. 16 -
4. Mr. Montez is a patient with type I diabetes...Ch. 16 - Prob. 5AYKBCh. 16 - You have read that aldosterone causes sodium ion...
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