PRESCOTT'S MICROBIO W/PROCTORIO
11th Edition
ISBN: 9781264731060
Author: WILLEY
Publisher: MCG
expand_more
expand_more
format_list_bulleted
Videos
Textbook Question
Chapter 16, Problem 4AL
Suppose that you carried out a U-tube experiment with two auxotrophs and discovered that recombination was not blocked by the filter but was stopped by treatment with deoxyribonuclease. What gene transfer process is responsible? Why would it be best to use double or triple auxotrophs in this experiment?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Time mapping is performed in a cross involving the genes his, leu, mal, and xyl. The recipient cells were auxotrophic for all four genes. After 25 minutes, mating was interrupted with the following results in recipient cells. Diagram the positions of these genes relative to the origin (O) of the F factor and to one another. (a) 90% were xyl+ (b) 80% were mal+ (c) 20% were his+ (d) none were leu+
The figure below shows the life cycle of the fungus Neurospora. The adult stage of the Neurospora is a multicellular haploid.
b) Neurospora has an arginine amino acid synthesis pathway shown below. Suppose I take the strain above that only grows with arginine supplements and cross it to a different mutant Neurospora strain that grows with arginine and citrulline supplements but not with ornithine supplements. Assuming gens A, B, and C are unlinked and there is only one mutation per stain:
What percentage of the progeny will grow on ornithine?
What percentage on citrulline?
What percentage on arginine?
1) Complementation occurs when auxotrophic organisms provide a wildtype allele that another auxotroph is lacking to produce a needed molecule for a chemical pathway. The wt allele can be passed on through horizontal gene flow, or the interaction of the auxotrophs during reproduction produces an offspring with a 'rescued' phenotype due to independent assortment returning favorable recombination of function alleles. If a researcher interacts four auxotrophs, and mutant -1 complements mutants -2, -3, and -4, and mutant -2 complements mutants -3 and -4, and mutant -3 complements -4, how many genes are indicated?
a) Four genes!
b) One gene with four alleles!
c) Three genes with one allele.
d)A complementation chart was not provided so I cannot answer this question.
2)
An inheritance ratio of 12:3:4 is a sure indication of:
a) Incomplete assortment.
b) A dihybrid example of codominance.
c) Dominant epistasis.
d) Recessive epistasis.
Chapter 16 Solutions
PRESCOTT'S MICROBIO W/PROCTORIO
Ch. 16.1 - Retrieve, Infer, Apply List three ways in which...Ch. 16.1 - Compare and contrast the means by which the...Ch. 16.1 - Give examples of intragenic and extragenic...Ch. 16.1 - Retrieve, Infer, Apply Sometimes a point mutation...Ch. 16.1 - Retrieve, Infer, Apply Why might a missense...Ch. 16.2 - How would you screen for a tryptophan auxotroph?...Ch. 16.2 - Why is a small amount of histidine added to the...Ch. 16.2 - Retrieve, Infer, Apply Describe how replica...Ch. 16.2 - Retrieve, Infer, Apply Why are mutant selection...Ch. 16.2 - Retrieve, Infer, Apply Briefly discuss how...
Ch. 16.2 - Describe how you would isolate a mutant that...Ch. 16.2 - Prob. 5CCCh. 16.3 - How is mismatch repair similar to DNA polymerase...Ch. 16.3 - How is damaged DNA recognized by the UvrAB...Ch. 16.3 - Prob. 1CCCh. 16.3 - Retrieve, Infer, Apply What role does DNA...Ch. 16.3 - Retrieve, Infer, Apply When E. coli cells are...Ch. 16.3 - Explain how the following DNA alterations and...Ch. 16.4 - An antibiotic-resistance gene located on a...Ch. 16.4 - What four fates can DNA have after entering a...Ch. 16.4 - How does homologous recombination differ from...Ch. 16.5 - What features are common to all types of...Ch. 16.5 - How does a transposon differ from an insertion...Ch. 16.5 - What is simple (cut-and-paste) transposition? What...Ch. 16.5 - What effect would you expect the existence of...Ch. 16.6 - Prob. 1MICh. 16.6 - What is bacterial conjugation and how was it...Ch. 16.6 - For F+, Hfr, and F strains of E. coli, indicate...Ch. 16.6 - Describe how F+ F and Hfr conjugation processes...Ch. 16.6 - Compare and contract F+ F and F F conjugation.Ch. 16.7 - According to this model, what would happen if DNA...Ch. 16.7 - Prob. 1CCCh. 16.7 - Describe how transformation occurs in S....Ch. 16.7 - Discuss two ways in which artificial...Ch. 16.8 - Compare the number of transducing particles that...Ch. 16.8 - Why cant the gal and bio genes be transduced by...Ch. 16.8 - Describe generalized transduction and how it...Ch. 16.8 - What is specialized transduction and how does it...Ch. 16.8 - How might one tell whether horizontal gene...Ch. 16.8 - Why doesnt a cell lyse after successful...Ch. 16.8 - Describe how conjugation, transformation, and...Ch. 16.9 - As a replicative transposon, what would happen if...Ch. 16 - Prob. 1RCCh. 16 - Prob. 2RCCh. 16 - Prob. 3RCCh. 16 - Prob. 4RCCh. 16 - Prob. 5RCCh. 16 - Prob. 6RCCh. 16 - Mutations are often considered harmful. Give an...Ch. 16 - Mistakes made during transcription affect the cell...Ch. 16 - Suppose that transduction took place when a U-tube...Ch. 16 - Suppose that you carried out a U-tube experiment...Ch. 16 - Prob. 5ALCh. 16 - Prob. 6ALCh. 16 - Prob. 7AL
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Which gene encodes the enzyme at the upper boxed step in the pathway? Which gene encodes the enzyme at the upper boxed step in the pathway? gene 1 gene 2 gene 3 gene 4arrow_forwardThe diploid that resulted from mating these two haploids would have which of the following auxotrophies? Select all that apply.Strain 1: MATa ura3-52 met15Δ0 leu2Δ0 Strain 2: MATalpha ura3-52 met15Δ0 trp1Δ63 a.) histidine b.) tryptophan c.) uracil d.) lysine e.) leucine f.) methioninearrow_forwardTime mapping is performed in a cross involving the genes his,leu, mal, and xyl. The recipient cells are auxotrophic for all fourgenes. After 25 minutes, mating is interrupted, with the resultsin recipient cells shown below. Diagram the positions of thesegenes relative to the origin (O) of the F factor and to one another.(a) 90% are xyl+(b) 80% are mal+(c) 20% are his+(d) None are leu+arrow_forward
- I breed a red flower to a blue flower. What would I get if the red and blue genes in these flowers were:A) incomplete dominance B) codominance 2. What are the two purposes of the membrane?3. What is the difference between passive and active transport? 4. What kind of stem cells are pluripotent?arrow_forwardYou identify an auxotroph that doesn't grow in normal medium, but does grow in medium supplemented with Q, indicating that it has a defect in one or more enzymes in this pathway. This auxotroph is also able to grow if you supplement the medium with D. Does this data support the one gene-one enzyme hypothesis? a yes, because only one enzyme is disrupted by the mutation b yes, because only one enzyme in the pathway is functional in the mutant strain c no, because multiple enzymes in the pathway could be functional in the mutant strain d no, because multiple enzymes could be disrupted by the mutationarrow_forwardThere is a group of six or so genes, called luxlux genes, whose gene products are necessary for light formation. Given that these bacterial genes are regulated together, propose a hypothesis for how the genes are organized and regulated. Match the terms in the left column to the appropriate blanks in the sentences on the right. Terms can be used once, more than once, or not at all. Terms: metaboliozed, density, quorum sensing, state, signal amplification, inhibit, activate, detected, secrete, transcrption initiation complex, consume, operon Bacterial cells blank molecules that can be blank by other bacterial cells. Sensing the concentration of such signalling molecules allows bacteria to monitor the local blank of cells, a phenomenon called blank. There is a group of genes in the genome of the described bacterium, called luxgenes, which are organized in an blank. Reaching a certain concentration, molecules involved in this process blank the luxgenes. As a result, the…arrow_forward
- In this activity you will analyze the results of experiments that investigate nutritional requirement of several mutant strains of yeast. The mutations in these strains cause a nutritional requirement for an amino acid, such that the strains will not grow in media that lack one specific amino acid. Any mutant that has a nutritional requirement is called an auxotroph, and is incapable of growing in a "minimal medium" containing only a carbon source (e.g., glucose), a simple nitrogen source (e.g., ammonium sulfate), and various salts and minerals. Such strains can be supported on a medium supplemented with only the missing nutrient or on a "rich" medium that contains amino acids, vitamins, nitrogenous bases, etc. (often in the form of an extract from yeast). The wild-type individual that can synthesize the metabolic component is a prototroph, and is capable of growth on minimal medium. The mutant strains in this activity are unable to synthesize tryptophan, lysine, or histidine; one…arrow_forwardThe figure below shows the life cycle of the fungus Neurospora. The adult stage of the Neurospora is a multicellular haploid: a. Between which two stages of the Neurospora life cycle do most mitotic cell divisions occur? b. Neurospora has an arginine amino acid synthesis pathway shown below: Suppose I have a neurospora strain that has a mutation such that it will not grow unless I supplement the media (food) with arginine (but not with citrulline or ornithine). What gene is mutated? Explain your reasoning. c. Suppose I take the strain above that only grows with arginine supplements and cross it to a different mutant Neurospora strain that grows with arginine and citrulline supplements but not ornithine supplements. Assming genes A, B and C are unlinked and there is only one mutation per strain: i) What percentage of the progeny will grow on ornithine? ii) What percentage on citrulline? iii) What percentage on arginine? Show your work for i), ii) and iii). [Can be answered in less…arrow_forwardA cross was performed between a yeast strain that requires methionine and lysine for growth (met− lys−)and another yeast strain, which is met+ lys+. One hundred asci were dissected, and colonies were grownfrom the four spores in each ascus. Cells from thesecolonies were tested for their ability to grow on petriplates containing either minimal medium (min), min+ lysine (lys), min + methionine (met), or min + lys+ met. The asci could be divided into two groupsbased on this analysis:Group 1: In 89 asci, cells from two of the four spore colonies couldgrow on all four kinds of media, while the other two spore coloniescould grow only on min + lys + met.Group 2: In 11 asci, cells from one of the four spore colonies couldgrow on all four kinds of petri plates. Cells from a second one ofthe four spore colonies could grow only on min + lys plates andon min + lys + met plates. Cells from a third of the four sporecolonies could only grow on min + met plates and on min + lys+ met. Cells from the…arrow_forward
- When two plants belonging to the same genus but different species are crossed, the F1 hybrid is more viable and has more ornate flowers. Unfortunately, this hybrid is sterile and can only be propagated by vegetative cuttings. Explain the sterility of the hybrid and what would have to occur for the sterility of this hybrid to be reversed.arrow_forwardYou self an F1 plant that is heterozygous for two dominant resistance genes, R1 and R2 and inoculate the F2 progeny with a pathogen race that is avirulent on both R1 and R2 (it carries both avirulence genes Avr1 and Avr2).You get 40 susceptible progeny out of 200 total progeny. (a) What is the linkage phase for these two genes? (b) What is the recombination distance?arrow_forwardA botanist wanted to see if a new strain of corn could germinate in soil that was too salty for regular corn. She conducted a study on the germination success of seeds from the new strain that were exposed to various levels of salty soil, from zero to normal (100mg/L) to high (200mg/L) to very high (400mg/L) to normally lethal (800mg/L). a.) Write a formal hypothesis for this study b.) Does her data support or reject the hypothesis? Explainarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Biology: The Dynamic Science (MindTap Course List)BiologyISBN:9781305389892Author:Peter J. Russell, Paul E. Hertz, Beverly McMillanPublisher:Cengage Learning
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Cengage Learning
Embryology | Fertilization, Cleavage, Blastulation; Author: Ninja Nerd;https://www.youtube.com/watch?v=8-KF0rnhKTU;License: Standard YouTube License, CC-BY