ND STONY BROOK UNIVERSITY LOOSELEAF GENETICS: FROM GENES TO GENOMES
ND STONY BROOK UNIVERSITY LOOSELEAF GENETICS: FROM GENES TO GENOMES
6th Edition
ISBN: 9781260406092
Author: HARTWELL, Leland, HOOD, Leroy, Goldberg, Michael
Publisher: Mcgraw-hill Education/stony Brook University
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Chapter 16, Problem 38P
Summary Introduction

a.

To determine:

The number of genes and operons depicted in the figure, the average gene density, and the representativeness of the value to the most bacterial genomes.

Introduction:

The mentioned figure shows the RNA-Seq results from the RNA-Seq experiment. In the analysis part, the computer lines up each of cDNA sequence read with the genome sequence of the bacterium.

Summary Introduction

b.

To determine:

Whether the given data proves that t2110 is nonfunctional and the process by which scientists were able to assign a direction to the transcription when no transcript of the gene was found.

Introduction:

In the performed experiment, it was found that no transcripts for the gene t2110 were detected.

Summary Introduction

c.

To determine:

The evidence that suggests the existence of an attenuation or riboswitch mechanism, causing premature transcriptional termination of an operon and the observation of any of the operon that might be controlled by such a mechanism.

Introduction:

The process of attenuation is considered as the method of control in some bacterial operons, and it causes premature termination of transcription.

Summary Introduction

d.

To determine:

The depiction of the given figure if the antisense mechanism was involved in controlling the gene or operon.

Introduction:

The mentioned figure does not provide any evidence that any of the genes or operons are regulated by an antisense transcript.

Summary Introduction

e.

To determine:

The possibility of the transcription of the galM gene from the different promoter.

Introduction:

The given figure depicts galM gene to be a part of the operon containing galETK. There is even a possibility that galM is transcribed from a separate promoter.

Summary Introduction

f.

To determine:

Whether the data shown in the figure reveal the presence of a regulatory mechanism in relation to an sRNA occluding the ribosome binding site.

Introduction:

The ribosome binding site is present in upstream to the start codon. It is a sequence of nucleotides which play an important role in recruiting the ribosome at the initiation of the translation process.

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Chapter 16 Solutions

ND STONY BROOK UNIVERSITY LOOSELEAF GENETICS: FROM GENES TO GENOMES

Ch. 16 - Mutants were isolated in which the constitutive...Ch. 16 - Suppose you have six strains of E. coli. One is...Ch. 16 - The previous problem raises some interesting...Ch. 16 - For each of the E. coli strains containing the lac...Ch. 16 - For each of the following growth conditions, what...Ch. 16 - For each of the following mutant E. coli strains,...Ch. 16 - Maltose utilization in E. coli requires the...Ch. 16 - Seven E. coli mutants were isolated. The activity...Ch. 16 - Cells containing missense mutations in the crp...Ch. 16 - Six strains of E.coli mutants 16 that had one of...Ch. 16 - a. The original constitutive operator mutations in...Ch. 16 - In an effort to determine the location of an...Ch. 16 - Prob. 23PCh. 16 - The footprinting experiment described in Fig....Ch. 16 - Why is the trp attenuation mechanism unique to...Ch. 16 - a. How many ribosomes are required at a minimum...Ch. 16 - The following is a sequence of the leader region...Ch. 16 - For each of the E. coli strains that follow,...Ch. 16 - Prob. 29PCh. 16 - For each element in the list that follows,...Ch. 16 - Among the structurally simplest riboswitches are...Ch. 16 - Great variation exists in the mechanisms by which...Ch. 16 - Many genes whose expression is turned on by DNA...Ch. 16 - In 2005, Frederick Blattner and his colleagues...Ch. 16 - The E.coli MalT protein is a positive regulator of...Ch. 16 - Prob. 36PCh. 16 - Prob. 37PCh. 16 - Prob. 38PCh. 16 - Prob. 39PCh. 16 - Prob. 40PCh. 16 - Prob. 41PCh. 16 - The researchers who investigated bioluminescence...Ch. 16 - Prob. 43PCh. 16 - Quorum sensing controls the expression of...Ch. 16 - Scientists are currently screening a chemical...
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Bacterial Genomics and Metagenomics; Author: Quadram Institute;https://www.youtube.com/watch?v=_6IdVTAFXoU;License: Standard youtube license