The process that can be performed to distinguish the genes involved in general stress response and the ones specific for osmolarity change. Introduction: One culture of E.coli is grown in a medium with high osmolarity, and another culture of E.coli is grown in a medium with low osmolarity to find the genes that are turned on or off in response to changes in osmolarity. RNA-seq analysis was performed for each culture. It was then hypothesized that osmotic changes might induce a general stress response that may be seen with other stressors like heat shock.

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Chapter 16, Problem 37P

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To determine:

The process that can be performed to distinguish the genes involved in general stress response and the ones specific for osmolarity change.

Introduction:

One culture of E.coli is grown in a medium with high osmolarity, and another culture of E.coli is grown in a medium with low osmolarity to find the genes that are turned on or off in response to changes in osmolarity. RNA-seq analysis was performed for each culture. It was then hypothesized that osmotic changes might induce a general stress response that may be seen with other stressors like heat shock.

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