ND STONY BROOK UNIVERSITY LOOSELEAF GENETICS: FROM GENES TO GENOMES
ND STONY BROOK UNIVERSITY LOOSELEAF GENETICS: FROM GENES TO GENOMES
6th Edition
ISBN: 9781260406092
Author: HARTWELL, Leland, HOOD, Leroy, Goldberg, Michael
Publisher: Mcgraw-hill Education/stony Brook University
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Chapter 16, Problem 43P
Summary Introduction

a.

To determine:

The reason behind the appearance of white color of luxR/lacZ colonies in the absence of autoinducer.

Introduction:

In an experiment to understand the molecular mechanism of quorum sensing, the use of two transcriptional fusion reporter genes was involved each having the 9 kb fragment of Vibrio fischeri DNA.

Summary Introduction

b.

To determine:

The reason behind the appearance of white color of luxICDABE/lacZ colonies in the absence of autoinducer.

Introduction:

In a reporter known as luxICDABE/lacZ, the luxICDABE operon regulatory sequences drive lacZ expression. It means that the structural genes of the operon are replaced by the lacZ coding sequences.

Summary Introduction

c.

To determine:

The reason behind the appearance of white color of luxR/lacZ colonies in the presence of autoinducer.

Introduction:

In the reporter known as luxR/lacZ, the luxR regulatory region drives the transcription of lacZ. It means that the luxR coding sequences are replaced by the lacZ.

Summary Introduction

d.

To determine:

The reason behind the appearance of blue colored luxICDABE/lacZ colonies in the presence of autoinducer and the time-dependency of the reaction.

Introduction:

The E.coli that contains either of the reporter (luxR/lacZ or luxICDABE/lacZ) has white colonies. When the purified autoinducer is added to the media, the luxR/lacZ colonies remain white but the luxICDABE/lacZ colonies turn blue over time.

Summary Introduction

e.

To determine:

The inference that can be made out about the transcription of the luxR gene from the given results.

Introduction:

The regulatory region of luxR drives the transcription of the lacZ gene. It indicates that the coding sequence of luxR is replaced by the coding sequence of lacZ.

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Chapter 16 Solutions

ND STONY BROOK UNIVERSITY LOOSELEAF GENETICS: FROM GENES TO GENOMES

Ch. 16 - Mutants were isolated in which the constitutive...Ch. 16 - Suppose you have six strains of E. coli. One is...Ch. 16 - The previous problem raises some interesting...Ch. 16 - For each of the E. coli strains containing the lac...Ch. 16 - For each of the following growth conditions, what...Ch. 16 - For each of the following mutant E. coli strains,...Ch. 16 - Maltose utilization in E. coli requires the...Ch. 16 - Seven E. coli mutants were isolated. The activity...Ch. 16 - Cells containing missense mutations in the crp...Ch. 16 - Six strains of E.coli mutants 16 that had one of...Ch. 16 - a. The original constitutive operator mutations in...Ch. 16 - In an effort to determine the location of an...Ch. 16 - Prob. 23PCh. 16 - The footprinting experiment described in Fig....Ch. 16 - Why is the trp attenuation mechanism unique to...Ch. 16 - a. How many ribosomes are required at a minimum...Ch. 16 - The following is a sequence of the leader region...Ch. 16 - For each of the E. coli strains that follow,...Ch. 16 - Prob. 29PCh. 16 - For each element in the list that follows,...Ch. 16 - Among the structurally simplest riboswitches are...Ch. 16 - Great variation exists in the mechanisms by which...Ch. 16 - Many genes whose expression is turned on by DNA...Ch. 16 - In 2005, Frederick Blattner and his colleagues...Ch. 16 - The E.coli MalT protein is a positive regulator of...Ch. 16 - Prob. 36PCh. 16 - Prob. 37PCh. 16 - Prob. 38PCh. 16 - Prob. 39PCh. 16 - Prob. 40PCh. 16 - Prob. 41PCh. 16 - The researchers who investigated bioluminescence...Ch. 16 - Prob. 43PCh. 16 - Quorum sensing controls the expression of...Ch. 16 - Scientists are currently screening a chemical...
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