a. To determine: The reason behind the appearance of white color of luxR/lacZ colonies in the absence of autoinducer. Introduction: In an experiment to understand the molecular mechanism of quorum sensing, the use of two transcriptional fusion reporter genes was involved each having the 9 kb fragment of Vibrio fischeri DNA.

Question

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Answer 1

Question

Chapter 16, Problem 43P

Summary Introduction

a.

To determine:

The reason behind the appearance of white color of luxR/lacZ colonies in the absence of autoinducer.

Introduction:

In an experiment to understand the molecular mechanism of quorum sensing, the use of two transcriptional fusion reporter genes was involved each having the 9 kb fragment of Vibrio fischeri DNA.

Summary Introduction

b.

To determine:

The reason behind the appearance of white color of luxICDABE/lacZ colonies in the absence of autoinducer.

Introduction:

In a reporter known as luxICDABE/lacZ, the luxICDABE operon regulatory sequences drive lacZ expression. It means that the structural genes of the operon are replaced by the lacZ coding sequences.

Summary Introduction

c.

To determine:

The reason behind the appearance of white color of luxR/lacZ colonies in the presence of autoinducer.

Introduction:

In the reporter known as luxR/lacZ, the luxR regulatory region drives the transcription of lacZ. It means that the luxR coding sequences are replaced by the lacZ.

Summary Introduction

d.

To determine:

The reason behind the appearance of blue colored luxICDABE/lacZ colonies in the presence of autoinducer and the time-dependency of the reaction.

Introduction:

The E.coli that contains either of the reporter (luxR/lacZ or luxICDABE/lacZ) has white colonies. When the purified autoinducer is added to the media, the luxR/lacZ colonies remain white but the luxICDABE/lacZ colonies turn blue over time.

Summary Introduction

e.

To determine:

The inference that can be made out about the transcription of the luxR gene from the given results.

Introduction:

The regulatory region of luxR drives the transcription of the lacZ gene. It indicates that the coding sequence of luxR is replaced by the coding sequence of lacZ.

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13. Practice Calculations: 3 colonies were suspended in the following dilution series and then a viable plate count and microscope count was performed. Calculate IDF's, TDF's and then calculate the CFU/mL in each tube by both methods. Finally calculate the cells in 1 colony by both methods. Show all of your calculations in the space provided on the following pages. 3 colonies 56 cells 10 μL 10 μL 100 μL 500 με m OS A B D 5.0 mL 990 με 990 με 900 με 500 μL EN 2 100 με 100 μL 118 colonies 12 colonies

Answer 2

Question

Chapter 16, Problem 43P

Summary Introduction

a.

To determine:

The reason behind the appearance of white color of luxR/lacZ colonies in the absence of autoinducer.

Introduction:

In an experiment to understand the molecular mechanism of quorum sensing, the use of two transcriptional fusion reporter genes was involved each having the 9 kb fragment of Vibrio fischeri DNA.

Summary Introduction

b.

To determine:

The reason behind the appearance of white color of luxICDABE/lacZ colonies in the absence of autoinducer.

Introduction:

In a reporter known as luxICDABE/lacZ, the luxICDABE operon regulatory sequences drive lacZ expression. It means that the structural genes of the operon are replaced by the lacZ coding sequences.

Summary Introduction

c.

To determine:

The reason behind the appearance of white color of luxR/lacZ colonies in the presence of autoinducer.

Introduction:

In the reporter known as luxR/lacZ, the luxR regulatory region drives the transcription of lacZ. It means that the luxR coding sequences are replaced by the lacZ.

Summary Introduction

d.

To determine:

The reason behind the appearance of blue colored luxICDABE/lacZ colonies in the presence of autoinducer and the time-dependency of the reaction.

Introduction:

The E.coli that contains either of the reporter (luxR/lacZ or luxICDABE/lacZ) has white colonies. When the purified autoinducer is added to the media, the luxR/lacZ colonies remain white but the luxICDABE/lacZ colonies turn blue over time.

Summary Introduction

e.

To determine:

The inference that can be made out about the transcription of the luxR gene from the given results.

Introduction:

The regulatory region of luxR drives the transcription of the lacZ gene. It indicates that the coding sequence of luxR is replaced by the coding sequence of lacZ.

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Answer 3

Question

Chapter 16, Problem 43P

Summary Introduction

a.

To determine:

The reason behind the appearance of white color of luxR/lacZ colonies in the absence of autoinducer.

Introduction:

In an experiment to understand the molecular mechanism of quorum sensing, the use of two transcriptional fusion reporter genes was involved each having the 9 kb fragment of Vibrio fischeri DNA.

Summary Introduction

b.

To determine:

The reason behind the appearance of white color of luxICDABE/lacZ colonies in the absence of autoinducer.

Introduction:

In a reporter known as luxICDABE/lacZ, the luxICDABE operon regulatory sequences drive lacZ expression. It means that the structural genes of the operon are replaced by the lacZ coding sequences.

Summary Introduction

c.

To determine:

The reason behind the appearance of white color of luxR/lacZ colonies in the presence of autoinducer.

Introduction:

In the reporter known as luxR/lacZ, the luxR regulatory region drives the transcription of lacZ. It means that the luxR coding sequences are replaced by the lacZ.

Summary Introduction

d.

To determine:

The reason behind the appearance of blue colored luxICDABE/lacZ colonies in the presence of autoinducer and the time-dependency of the reaction.

Introduction:

The E.coli that contains either of the reporter (luxR/lacZ or luxICDABE/lacZ) has white colonies. When the purified autoinducer is added to the media, the luxR/lacZ colonies remain white but the luxICDABE/lacZ colonies turn blue over time.

Summary Introduction

e.

To determine:

The inference that can be made out about the transcription of the luxR gene from the given results.

Introduction:

The regulatory region of luxR drives the transcription of the lacZ gene. It indicates that the coding sequence of luxR is replaced by the coding sequence of lacZ.

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Answer 4

Question

Chapter 16, Problem 43P

Summary Introduction

a.

To determine:

The reason behind the appearance of white color of luxR/lacZ colonies in the absence of autoinducer.

Introduction:

In an experiment to understand the molecular mechanism of quorum sensing, the use of two transcriptional fusion reporter genes was involved each having the 9 kb fragment of Vibrio fischeri DNA.

Summary Introduction

b.

To determine:

The reason behind the appearance of white color of luxICDABE/lacZ colonies in the absence of autoinducer.

Introduction:

In a reporter known as luxICDABE/lacZ, the luxICDABE operon regulatory sequences drive lacZ expression. It means that the structural genes of the operon are replaced by the lacZ coding sequences.

Summary Introduction

c.

To determine:

The reason behind the appearance of white color of luxR/lacZ colonies in the presence of autoinducer.

Introduction:

In the reporter known as luxR/lacZ, the luxR regulatory region drives the transcription of lacZ. It means that the luxR coding sequences are replaced by the lacZ.

Summary Introduction

d.

To determine:

The reason behind the appearance of blue colored luxICDABE/lacZ colonies in the presence of autoinducer and the time-dependency of the reaction.

Introduction:

The E.coli that contains either of the reporter (luxR/lacZ or luxICDABE/lacZ) has white colonies. When the purified autoinducer is added to the media, the luxR/lacZ colonies remain white but the luxICDABE/lacZ colonies turn blue over time.

Summary Introduction

e.

To determine:

The inference that can be made out about the transcription of the luxR gene from the given results.

Introduction:

The regulatory region of luxR drives the transcription of the lacZ gene. It indicates that the coding sequence of luxR is replaced by the coding sequence of lacZ.

Expert Solution & Answer

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Answer 5

Chapter 16, Problem 43P

Summary Introduction

a.

To determine:

The reason behind the appearance of white color of luxR/lacZ colonies in the absence of autoinducer.

Introduction:

In an experiment to understand the molecular mechanism of quorum sensing, the use of two transcriptional fusion reporter genes was involved each having the 9 kb fragment of Vibrio fischeri DNA.

Summary Introduction

b.

To determine:

The reason behind the appearance of white color of luxICDABE/lacZ colonies in the absence of autoinducer.

Introduction:

In a reporter known as luxICDABE/lacZ, the luxICDABE operon regulatory sequences drive lacZ expression. It means that the structural genes of the operon are replaced by the lacZ coding sequences.

Summary Introduction

c.

To determine:

The reason behind the appearance of white color of luxR/lacZ colonies in the presence of autoinducer.

Introduction:

In the reporter known as luxR/lacZ, the luxR regulatory region drives the transcription of lacZ. It means that the luxR coding sequences are replaced by the lacZ.

Summary Introduction

d.

To determine:

The reason behind the appearance of blue colored luxICDABE/lacZ colonies in the presence of autoinducer and the time-dependency of the reaction.

Introduction:

The E.coli that contains either of the reporter (luxR/lacZ or luxICDABE/lacZ) has white colonies. When the purified autoinducer is added to the media, the luxR/lacZ colonies remain white but the luxICDABE/lacZ colonies turn blue over time.

Summary Introduction

e.

To determine:

The inference that can be made out about the transcription of the luxR gene from the given results.

Introduction:

The regulatory region of luxR drives the transcription of the lacZ gene. It indicates that the coding sequence of luxR is replaced by the coding sequence of lacZ.

Answer 6

Chapter 16, Problem 43P

Summary Introduction

a.

To determine:

The reason behind the appearance of white color of luxR/lacZ colonies in the absence of autoinducer.

Introduction:

In an experiment to understand the molecular mechanism of quorum sensing, the use of two transcriptional fusion reporter genes was involved each having the 9 kb fragment of Vibrio fischeri DNA.

Answer 7

Chapter 16, Problem 43P

Summary Introduction

a.

To determine:

The reason behind the appearance of white color of luxR/lacZ colonies in the absence of autoinducer.

Introduction:

In an experiment to understand the molecular mechanism of quorum sensing, the use of two transcriptional fusion reporter genes was involved each having the 9 kb fragment of Vibrio fischeri DNA.

Answer 8

Summary Introduction

b.

To determine:

The reason behind the appearance of white color of luxICDABE/lacZ colonies in the absence of autoinducer.

Introduction:

In a reporter known as luxICDABE/lacZ, the luxICDABE operon regulatory sequences drive lacZ expression. It means that the structural genes of the operon are replaced by the lacZ coding sequences.

Answer 9

Summary Introduction

b.

To determine:

The reason behind the appearance of white color of luxICDABE/lacZ colonies in the absence of autoinducer.

Introduction:

In a reporter known as luxICDABE/lacZ, the luxICDABE operon regulatory sequences drive lacZ expression. It means that the structural genes of the operon are replaced by the lacZ coding sequences.

Answer 10

Summary Introduction

c.

To determine:

The reason behind the appearance of white color of luxR/lacZ colonies in the presence of autoinducer.

Introduction:

In the reporter known as luxR/lacZ, the luxR regulatory region drives the transcription of lacZ. It means that the luxR coding sequences are replaced by the lacZ.

Answer 11

Summary Introduction

c.

To determine:

The reason behind the appearance of white color of luxR/lacZ colonies in the presence of autoinducer.

Introduction:

In the reporter known as luxR/lacZ, the luxR regulatory region drives the transcription of lacZ. It means that the luxR coding sequences are replaced by the lacZ.

Answer 12

Summary Introduction

d.

To determine:

The reason behind the appearance of blue colored luxICDABE/lacZ colonies in the presence of autoinducer and the time-dependency of the reaction.

Introduction:

The E.coli that contains either of the reporter (luxR/lacZ or luxICDABE/lacZ) has white colonies. When the purified autoinducer is added to the media, the luxR/lacZ colonies remain white but the luxICDABE/lacZ colonies turn blue over time.

Answer 13

Summary Introduction

d.

To determine:

The reason behind the appearance of blue colored luxICDABE/lacZ colonies in the presence of autoinducer and the time-dependency of the reaction.

Introduction:

The E.coli that contains either of the reporter (luxR/lacZ or luxICDABE/lacZ) has white colonies. When the purified autoinducer is added to the media, the luxR/lacZ colonies remain white but the luxICDABE/lacZ colonies turn blue over time.

Answer 14

Summary Introduction

e.

To determine:

The inference that can be made out about the transcription of the luxR gene from the given results.

Introduction:

The regulatory region of luxR drives the transcription of the lacZ gene. It indicates that the coding sequence of luxR is replaced by the coding sequence of lacZ.

Answer 15

Summary Introduction

e.

To determine:

The inference that can be made out about the transcription of the luxR gene from the given results.

Introduction:

The regulatory region of luxR drives the transcription of the lacZ gene. It indicates that the coding sequence of luxR is replaced by the coding sequence of lacZ.

Answer 16

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Answer 17

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Answer 18

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Answer 19

Ch. 16 - For each of the terms in the left column, choose...Ch. 16 - The following statement occurs early in this...Ch. 16 - One of the main lessons of this chapter is that...Ch. 16 - All mutations that abolish function of the Rho...Ch. 16 - The figure at the beginning of this chapter shows...Ch. 16 - The promoter of an operon is the site to which RNA...Ch. 16 - You are studying an operon containing three genes...Ch. 16 - You have isolated a protein that binds to DNA in...Ch. 16 - You have isolated two different mutants reg1 and...Ch. 16 - Bacteriophage , after infecting a cell, can...

Solution Summary: The author analyzes the molecular mechanism of quorum sensing using two transcriptional fusion reporter genes. In the absence of autoinducer, the luxICDABE operon regulatory sequences drive lacZ expression.

Concept explainers

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c.

To determine:

The reason behind the appearance of white color of luxR/lacZ colonies in the presence of autoinducer.

Introduction:

In the reporter known as luxR/lacZ, the luxR regulatory region drives the transcription of lacZ. It means that the luxR coding sequences are replaced by the lacZ.

e.

To determine:

The inference that can be made out about the transcription of the luxR gene from the given results.

Introduction:

The regulatory region of luxR drives the transcription of the lacZ gene. It indicates that the coding sequence of luxR is replaced by the coding sequence of lacZ.

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Chapter 16 Solutions

Solution Summary: The author analyzes the molecular mechanism of quorum sensing using two transcriptional fusion reporter genes. In the absence of autoinducer, the luxICDABE operon regulatory sequences drive lacZ expression.

Concept explainers

Videos

c. To determine: The reason behind the appearance of white color of luxR/lacZ colonies in the presence of autoinducer. Introduction: In the reporter known as luxR/lacZ, the luxR regulatory region drives the transcription of lacZ. It means that the luxR coding sequences are replaced by the lacZ.

e. To determine: The inference that can be made out about the transcription of the luxR gene from the given results. Introduction: The regulatory region of luxR drives the transcription of the lacZ gene. It indicates that the coding sequence of luxR is replaced by the coding sequence of lacZ.

Want to see the full answer?

Chapter 16 Solutions

c.

To determine:

The reason behind the appearance of white color of luxR/lacZ colonies in the presence of autoinducer.

Introduction:

In the reporter known as luxR/lacZ, the luxR regulatory region drives the transcription of lacZ. It means that the luxR coding sequences are replaced by the lacZ.

e.

To determine:

The inference that can be made out about the transcription of the luxR gene from the given results.

Introduction:

The regulatory region of luxR drives the transcription of the lacZ gene. It indicates that the coding sequence of luxR is replaced by the coding sequence of lacZ.