Chapter 15, Problem 9P

An example of a gene-targeting DNA plasmid vector for insertion of a transgene into chloroplast DNA by biolistic transformation is shown in the following diagram. The plasmid DNA can be prepared in large quantities in E. coli before being shot into plant cells with a gene gun. Match the component of the construct with its function. (RE1, RE2, and RE3 are different restriction enzyme recognition sites unique to the plasmid vector.)

 

a. spectinomycin	1. DNA that mediates resistance gene integration
b. cpDNA sequences	2. gene used to select chloroplast transformants
c. RE2	3. sequence for plasmid replication in E. coli
d. ori	4. sites at which targeting chloroplast DNA can be inserted into the vector
e. RE1 and RE3	5. site at which transgene can be inserted into the vector