EBK GENETICS: FROM GENES TO GENOMES
6th Edition
ISBN: 9781260041255
Author: HARTWELL
Publisher: MCGRAW HILL BOOK COMPANY
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Textbook Question
Chapter 15, Problem 6P
How do you know if the halibut you purchased at the supermarket is really halibut? To identify the source of a biological sample, scientists PCR amplify and then sequence a region of DNA known to vary between species. For animals, this DNA region is a 648–base pair portion of the mitochondrial cytochrome oxidase I gene. The sequence of this mtDNA region acts as a so-called DNA barcode because a database exists that contains the sequences of this mtDNA region that are unique for hundreds of thousands of animal species.
| a. | Why do you think that a region of mitochondrial DNA is used for barcoding animals, as opposed to a region of nuclear genomic DNA? |
| b. | A single pair of PCR primers can be used to barcode any species of fish. Explain how this is possible. |
| c. | List criteria that scientists would have considered when determining which mitochondrial DNA sequence to use for barcoding animals. |
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10. Your instructor will give you 2 amino acids during the activity session (video 2-7.
A. First color all the polar and non-polar covalent bonds in the R groups of your 2 amino acids
using the same colors as in #7. Do not color the bonds in the backbone of each amino acid.
B. Next, color where all the hydrogen bonds, hydrophobic interactions and ionic bonds could
occur in the R group of each amino acid. Use the same colors as in #7. Do not color the bonds
in the backbone of each amino acid.
C. Position the two amino acids on the page below in an orientation where the two R groups
could bond together. Once you are satisfied, staple or tape the amino acids in place and label
the bond that you formed between the two R groups.
- Polar covalent Bond - Red
- Non polar Covalent boND- yellow
- Ionic BonD - PINK
Hydrogen Bonn - Purple
Hydrophobic interaction-green
O=C-N
H
I.
H
HO
H
=O
CH2
C-C-N
HICK
H
HO
H
CH2
OH
H₂N
C = O
Find the dental formula and enter it in the following format:
I3/3 C1/1 P4/4 M2/3 = 42 (this is not the correct number, just the correct format)
Please be aware: the upper jaw is intact (all teeth are present). The bottom jaw/mandible is not intact. The front teeth should include 6 total rectangular teeth (3 on each side) and 2 total large triangular teeth (1 on each side).
Chapter 15 Solutions
EBK GENETICS: FROM GENES TO GENOMES
Ch. 15 - Match each numbered item with the most closely...Ch. 15 - Assuming human cells have on average 1000...Ch. 15 - Reverse translation is a term given to the process...Ch. 15 - The human nuclear genome encodes tRNAs with 32...Ch. 15 - The human mitochondrial genome includes no genes...Ch. 15 - How do you know if the halibut you purchased at...Ch. 15 - Is each of these statements true of chloroplast or...Ch. 15 - Suppose you are characterizing the DNA of a...Ch. 15 - An example of a gene-targeting DNA plasmid vector...Ch. 15 - Which of the following characteristics of...
Ch. 15 - The Saccharomyces cerevisiae nuclear gene ARG8...Ch. 15 - The so-called hypervariable regions HV1 and HV2 of...Ch. 15 - Suppose a new mutation arises in a mitochondrial...Ch. 15 - Describe at least two ways in which the...Ch. 15 - Why are severe mitochondrial or chloroplast gene...Ch. 15 - Suppose you are examining a newly found plant...Ch. 15 - A form of male sterility in corn is inherited...Ch. 15 - Plant breeders have long appreciated the...Ch. 15 - A mutant haploid strain of Saccharomyces...Ch. 15 - Prob. 20PCh. 15 - What characteristics in a human pedigree suggest a...Ch. 15 - The first person in the family represented by the...Ch. 15 - In 1988, neurologists in Australia reported the...Ch. 15 - If you were a genetic counselor and had a patient...Ch. 15 - Kearns-Sayre syndrome KSS, Pearson syndrome, and...Ch. 15 - Many clinically relevant mitochondrial diseases...Ch. 15 - Leigh syndrome is characterized by psychomotor...Ch. 15 - All mutations in mitochondrial genes ultimately...Ch. 15 - How could researchers have determined that the...
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