ND STONY BROOK UNIVERSITY LOOSELEAF GENETICS: FROM GENES TO GENOMES
6th Edition
ISBN: 9781260406092
Author: HARTWELL, Leland, HOOD, Leroy, Goldberg, Michael
Publisher: Mcgraw-hill Education/stony Brook University
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Chapter 15, Problem 19P
A mutant haploid strain of Saccharomyces cerevisiae (yeast) called cox2-1 was found that was unable to grow on media containing glycerol as the sole source of carbon and energy. (Glycerol is a nonfermentable substrate for yeast.) This strain could, however, grow on the fermentable substrate glucose. Researchers discovered that cox2-1 cells lack a mitochondrial protein called cytochrome c oxidase.
| a. | Explain why cox2-1 cells can grow on medium containing glucose but not on glycerol medium. |
| b. | When cox2-1 was crossed with a wild-type yeast strain and the resultant diploid cells were allowed to grow mitotically, it was found that about half the diploid clones were able to grow on glycerol, while the other half could not. The diploid clones that could grow on glycerol were induced to sporulate, and they yielded tetrads with four spores that were all able to grow on glycerol medium. In all of these tetrads, two of the haploid progeny were of mating type a and two of mating type α. The diploids that could not grow on glycerol could not sporulate. What do the results of the mating say about the location of the cox2-1 mutation? |
| c. | A different mutant strain of yeast called pet111-1 is also unable to grow on glycerol medium but still can grow on glucose medium. These mutant cells similarly lacked the cytochrome c oxidase. When pet111-1 was crossed with a wild-type haploid strain of the opposite mating type, the resultant diploids were able to grow on glycerol and yielded asci that all showed a 2:2 segregation of haploid cells that could or could not grow on glycerol. Explain these results in light of your answer to part (b) |
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A mutant haploid strain of Saccharomyces cerevisiae(yeast) called cox2-1 was found that was unable togrow on media containing glycerol as the sole sourceof carbon and energy. (Glycerol is a nonfermentablesubstrate for yeast.) This strain could, however, growon the fermentable substrate glucose. Researchers discovered that cox2-1 cells lack a mitochondrial proteincalled cytochrome c oxidase.a. Explain why cox2-1 cells can grow on mediumcontaining glucose but not on glycerol medium.
A mutant haploid strain of Saccharomyces cerevisiae(yeast) called cox2-1 was found that was unable togrow on media containing glycerol as the sole sourceof carbon and energy. (Glycerol is a nonfermentablesubstrate for yeast.) This strain could, however, growon the fermentable substrate glucose. Researchers discovered that cox2-1 cells lack a mitochondrial proteincalled cytochrome c oxidase.a. Explain why cox2-1 cells can grow on mediumcontaining glucose but not on glycerol medium.b. When cox2-1 was crossed with a wild-type yeaststrain and the resultant diploid cells were allowedto grow mitotically, it was found that about half thediploid clones were able to grow on glycerol, whilethe other half could not. The diploid clones thatcould grow on glycerol were induced to sporulate,and they yielded tetrads with four spores that wereall able to grow on glycerol medium. In all of thesetetrads, two of the haploid progeny were of matingtype a and two of mating type α. The diploids thatcould not…
Leigh syndrome is characterized by psychomotor regression: that is, the progressive loss of mental andmovement abilities. Patients also suffer from lacticacidosis, a condition in which mitochondrial respiration is deficient, so their tissues metabolize glucoseanaerobically, leading to the buildup of lactate. Somepatients with Leigh syndrome have a mutation in themitochondrial gene MT-CO3, which encodes a subunit of the electron transport complex cytochromec oxidase. Other patients diagnosed with Leigh syndrome have a loss-of-function mutation in the nucleargene SURF1, which encodes a factor needed for theassembly of this same enzyme complex.a. How can the same symptoms result from mutationsin a mitochondrial gene and from mutations in anuclear gene?
Chapter 15 Solutions
ND STONY BROOK UNIVERSITY LOOSELEAF GENETICS: FROM GENES TO GENOMES
Ch. 15 - Match each numbered item with the most closely...Ch. 15 - Assuming human cells have on average 1000...Ch. 15 - Reverse translation is a term given to the process...Ch. 15 - The human nuclear genome encodes tRNAs with 32...Ch. 15 - The human mitochondrial genome includes no genes...Ch. 15 - How do you know if the halibut you purchased at...Ch. 15 - Is each of these statements true of chloroplast or...Ch. 15 - Suppose you are characterizing the DNA of a...Ch. 15 - An example of a gene-targeting DNA plasmid vector...Ch. 15 - Which of the following characteristics of...
Ch. 15 - The Saccharomyces cerevisiae nuclear gene ARG8...Ch. 15 - The so-called hypervariable regions HV1 and HV2 of...Ch. 15 - Suppose a new mutation arises in a mitochondrial...Ch. 15 - Describe at least two ways in which the...Ch. 15 - Why are severe mitochondrial or chloroplast gene...Ch. 15 - Suppose you are examining a newly found plant...Ch. 15 - A form of male sterility in corn is inherited...Ch. 15 - Plant breeders have long appreciated the...Ch. 15 - A mutant haploid strain of Saccharomyces...Ch. 15 - Prob. 20PCh. 15 - What characteristics in a human pedigree suggest a...Ch. 15 - The first person in the family represented by the...Ch. 15 - In 1988, neurologists in Australia reported the...Ch. 15 - If you were a genetic counselor and had a patient...Ch. 15 - Kearns-Sayre syndrome KSS, Pearson syndrome, and...Ch. 15 - Many clinically relevant mitochondrial diseases...Ch. 15 - Leigh syndrome is characterized by psychomotor...Ch. 15 - All mutations in mitochondrial genes ultimately...Ch. 15 - How could researchers have determined that the...
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