BIOL 107 LAB PKG W/LAB SG +ACCESS >IC<
BIOL 107 LAB PKG W/LAB SG +ACCESS >IC<
12th Edition
ISBN: 9781259827082
Author: Mader
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 14.3, Problem 2CYP
Summary Introduction

To discuss:

An example each for in vivo gene therapy and ex vivo gene therapy.

Introduction:

Gene therapy is of two kinds based on how the healthy genes are delivered, in vivo gene therapy and ex vivo gene therapy. In case of in vivo gene therapy the gene is directly put in the patient’s body while in ex vivo gene therapy the cells from the patient are removed, cultured, transformed and then put back in his/her body.

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a) Find a nitrogen base.  What carbon is the base attached to? b) What two nitrogenous bases are held together by 2 hydrogen bonds? c)What two nitrogenous bases are held together by 3 hydrogen bonds?
You will be building a double-stranded DNA molecule, representing a short (20 base) sequence that could be found in a chromosome.  It will have a 3' --> 5' strand and a 5' --> 3' strand. Use two full 12-base-pair DNA models.  a) Upload a photo of a model once the first 6 nucleotides have been inserted to base pair with the two parent strands. b)Upload a photo of DNA polymerase I and Ligase in "action" removing the RNA primer nucleotides and making new phosphodiester bonds. c)Upload a photo of the final two DNA strands, after Termination, identifying the parent and daughter strands of each.
Write down the 12 base-pairs (ex A-T or G-C) contained in the two models you will us from top to bottom below Model 1 bp:   Model 2 bp:
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