BIOLOGY-TEXT
BIOLOGY-TEXT
5th Edition
ISBN: 9781260169621
Author: BROOKER
Publisher: MCG
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Chapter 14.3, Problem 1CC
Summary Introduction

To determine: The effect on the function of RNA polymerase as a result of the interaction of activator with a mediator.

Introduction: A mediator is a protein complex that is required during the process of transcription in eukaryotes whereas activators are regulatory transcription factors that are required to regulate the transcription process.

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O Macmillan Learning Glu-His-Trp-Ser-Gly-Leu-Arg-Pro-Gly The pKa values for the peptide's side chains, terminal amino groups, and carboxyl groups are provided in the table. Amino acid Amino pKa Carboxyl pKa Side-chain pKa glutamate 9.60 2.34 histidine 9.17 1.82 4.25 6.00 tryptophan 9.39 2.38 serine 9.15 2.21 glycine 9.60 2.34 leucine 9.60 2.36 arginine 9.04 2.17 12.48 proline 10.96 1.99 Calculate the net charge of the molecule at pH 3. net charge at pH 3: Calculate the net charge of the molecule at pH 8. net charge at pH 8: Calculate the net charge of the molecule at pH 11. net charge at pH 11: Estimate the isoelectric point (pl) for this peptide. pl:
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