Biology
12th Edition
ISBN: 9781260494570
Author: Raven, Peter
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 14, Problem 3A
The Meselson and Stahl experiment used a density label to be able to
a. determine the directionality of
b. differentially label DNA and protein.
c. distinguish between newly replicated and old strands.
d. distinguish between replicated DNA and RNA primers.
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Place the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide.
B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube.
D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.
Which of the following statements BEST DESCRIBES the main findings of the Meselson-Stahl experiment?
A.
DNA can be separated using centrifugation
B.
The semiconservative model of DNA replication is more accurate than the dispersive or conservative models of DNA replication
C.
Using 14N in experiments is an effective way of tracking nitrogen molecules
D.
Bacteria grown in the presence of a heavier nitrogen isotope (15N) will replicate at a slower rate than those that utilise a lighter nitrogen isotope (14N)
E.
Both strands of each new DNA double helix are brand new and synthesized from individual nucleotides
The diagram illustrating the polymerase chain reaction (PCR) technique is provided below. How does the number of copies of the DNA region being amplified change at the end of each cycle of the polymerase chain reaction?
Group of answer choices
a. The number of copies triples (or triplicates).
b. The number of copies does not change.
c. The number of copies quadruples (or quadruplicates).
d. The number of copies doubles (or duplicates).
e. The number of copies halves.
Chapter 14 Solutions
Biology
Ch. 14.1 - Describe the experiments of Griffith and Avery.Ch. 14.1 - Evaluate the evidence for DNA as genetic material.Ch. 14.2 - Explain how the WatsonCrick structure rationalized...Ch. 14.2 - Prob. 2LOCh. 14.3 - Prob. 1LOCh. 14.3 - Prob. 2LOCh. 14.4 - Prob. 1LOCh. 14.4 - Prob. 2LOCh. 14.4 - Diagram the functions found at the replication...Ch. 14.5 - Compare eukaryotic replication with prokaryotic.
Ch. 14.5 - Prob. 2LOCh. 14.5 - Prob. 3LOCh. 14.6 - Prob. 1LOCh. 14.6 - Prob. 2LOCh. 14 - Prob. 1DACh. 14 - Prob. 2DACh. 14 - Prob. 1IQCh. 14 - Prob. 2IQCh. 14 - How does the structure of eukaryotic genomes...Ch. 14 - Prob. 4IQCh. 14 - Prob. 1UCh. 14 - Which of the following is NOT a component of DNA?...Ch. 14 - Chargaff studied the composition of DNA from...Ch. 14 - The bonds that hold two complementary strands of...Ch. 14 - Prob. 5UCh. 14 - Prob. 6UCh. 14 - Which of the following is NOT pan of the...Ch. 14 - If one strand of a DNA is 5 ATCGTTAAGCGAGTCA 3,...Ch. 14 - Hershey and Chase used radioactive phosphorus and...Ch. 14 - The Meselson and Stahl experiment used a density...Ch. 14 - Prob. 4ACh. 14 - If the activity of DNA ligase was removed from...Ch. 14 - Successful DNA synthesis requires all of the...Ch. 14 - The synthesis of telomeres a. uses DNA polymerase,...Ch. 14 - When mutations that affected DNA replication were...Ch. 14 - Prob. 1SCh. 14 - In the Meselson-Stahl experiment, a control...Ch. 14 - Enzyme function is critically important for the...
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- In the Meselson-Stahl experiment, what happened after the E. coli was moved to the N14 medium and two rounds of cell division occurred? This result demonstrated that DNA replicated in a semi-conservative manner. A. The DNA formed one band at the N15 density level and one band at the N14 density level. B. The DNA formed one band between the N14 and N15 density levels and one band at the N14 density level. .C. The DNA only formed one band between the N14 and N15 density levels.arrow_forwardWhich of the following best describes the process of DNA seqencing. a. DNA is seperated on a gel and the different bands are labled with flouroscent nucleotides and scanned with a laser. b. A laser is used to flurorescently label the nucleotides present with in the DNA , the DNA is run on a gel and then the DNA is droken into fragments c. Nucleotides are scanned with a laser and incrprorated into the DNA that has been seperated on a gel and then DNA is amplified with PCR. d. fragments of DNA are produced in a reaction that lables them with any of four different fluroscent dyes and the fragmented then are run on a gel and scanned with laser e. DNA is broken down into its constituents nucleotides and the nucleotides are then run on a gel and purified with a laserarrow_forwardHow does E. coli determine which nucleotide to "fix" in a base mismatch? The appropriate DNA repair system: A.recognizes fully methylated DNA and replaces the purine. B. recognizes partially methylated DNA and replaces the pyrimdine. C. Recognizes partially methylated DNA and fixes the methylated strand D. Recongnizes partially methylated DNA and fixes the nonmethylated strand E. Makes no distiction between strands, and therefore fixes the wrong base 50% of the timearrow_forward
- A.) Base excision repair requires polymerases. B.) In DNA repair by excision, the non-damaged strand is used as a template for a new strand of DNA. a. Statement A is correct b. Statement B is correct c. Both A and B are correct d. Both A and B are incorrectarrow_forwardWhat is Sanger sequencing? Why do we use ddNTP? How to read a DNA sequence gel? c. What is a cDNA seq (RNA seq)? d. What is the main difference between a genomic and a transcriptome study?arrow_forwardWhat is a restriction endonuclease? Select one: a. It is an enzyme that cleaves at a specific nucleotide sequence. b. It restricts the movement of the DNA outside the nucleus. c. It proofreads the DNA for accidental damages and corrects any errors. d. It is an enzyme that separates the DNA double helix.arrow_forward
- In gene mapping using generalized transduction, bacterial genes that are cotransduced are a. far apart on the bacterial chromosome. b. on different bacterial chromosomes. c. close together on the bacterial chromosome. d. on a plasmid.arrow_forwardSuppose the experiment of Meselson and Stahl was performed on a sample of 8 cells, each containing one copy of its circular double-stranded DNA genome, and that had been growing on normal 14N medium. You then grew the cells for 3 generations in medium containing 15N. The outcome would be A) 8 cells with single-stranded DNA molecules with 14N, and 24 cells with single-stranded DNA molecules with 15N. B) 16 cells with double-stranded DNA molecules with equal amounts of 14N and 15N, and 48 cells with double-stranded DNA molecules with 15N. C) 8 cells with double-stranded DNA molecules with equal amounts of 14N and 15N, and 24 cells with double-stranded DNA molecules with 15N. D) 8 cells with double-stranded DNA molecules with equal amounts of 14N and 15N, and 32 cells with double-stranded DNA molecules with 15N. E) 65 cells with single-stranded DNA molecules with 15N.arrow_forwardExplain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.arrow_forward
- How does the synthesis-dependent strand-annealing model differ from the double-strand break model of homologous recombination? a. The product is always noncrossover. b. There is no second strand capture. c. No Holliday junctions are formed. d. The strand in the D loop is displaced. e. All of these are correct.arrow_forwardWhich of the following is TRUE with respect to electrophoresis? a. DNA is not attracted to either pole but in fact moves by diffusion through the TAE buffer in the agarose. b. DNA can be attracted to either pole depending on the bases it is made of. c. DNA is attracted to the negative pole. d. DNA is attracted to the postive polearrow_forwardGive the purpose of gel electrophoresis system. If larger DNA is loaded in the gel, what will be the movement of such DNA, how about is smaller DNA is loaded into the gel?arrow_forward
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