Biology
12th Edition
ISBN: 9781260494570
Author: Raven, Peter
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Textbook Question
Chapter 14, Problem 2A
Hershey and Chase used radioactive phosphorus and sulfur to
a. label DNA and protein uniformly.
b. differentially label DNA and protein.
c. identify the transforming principle.
d. Both b and c are correct.
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Which of the following is true?
a.
In one technique, the DNA sequence can be determined using one strand of DNA as it passes through a nanopore.
b.
Next generation sequencing is slow but accurate.
c.
Both of the above are true.
Arrange the following steps in the sequence they would happen in a DNA cloning experiment.
a. sealing DNA fragments into vectors with DNA ligase;
b. utilizing a probe to detect a clone in the library;
c. sequencing the clone's DNA;
d. creating a DNA library of clones;
e. cutting genomic DNA with restriction enzymes.
A.
e,a,d,b,c
B.
a,d,b,c,e
C.
c,b,e,a,d
D.
e,d,a,c,b
Which of the following statements about DNA probes are false?
a. Probes can be labeled with a fluorescent dye to facilitate detection.
b. Probes can be labeled with radioactive phosphorus to facilitate detection.
c. Probes are typically composed of double stranded pieces of DNA.
d. Probes are typically composed of single stranded pieces of DNA.
Chapter 14 Solutions
Biology
Ch. 14.1 - Describe the experiments of Griffith and Avery.Ch. 14.1 - Evaluate the evidence for DNA as genetic material.Ch. 14.2 - Explain how the WatsonCrick structure rationalized...Ch. 14.2 - Prob. 2LOCh. 14.3 - Prob. 1LOCh. 14.3 - Prob. 2LOCh. 14.4 - Prob. 1LOCh. 14.4 - Prob. 2LOCh. 14.4 - Diagram the functions found at the replication...Ch. 14.5 - Compare eukaryotic replication with prokaryotic.
Ch. 14.5 - Prob. 2LOCh. 14.5 - Prob. 3LOCh. 14.6 - Prob. 1LOCh. 14.6 - Prob. 2LOCh. 14 - Prob. 1DACh. 14 - Prob. 2DACh. 14 - Prob. 1IQCh. 14 - Prob. 2IQCh. 14 - How does the structure of eukaryotic genomes...Ch. 14 - Prob. 4IQCh. 14 - Prob. 1UCh. 14 - Which of the following is NOT a component of DNA?...Ch. 14 - Chargaff studied the composition of DNA from...Ch. 14 - The bonds that hold two complementary strands of...Ch. 14 - Prob. 5UCh. 14 - Prob. 6UCh. 14 - Which of the following is NOT pan of the...Ch. 14 - If one strand of a DNA is 5 ATCGTTAAGCGAGTCA 3,...Ch. 14 - Hershey and Chase used radioactive phosphorus and...Ch. 14 - The Meselson and Stahl experiment used a density...Ch. 14 - Prob. 4ACh. 14 - If the activity of DNA ligase was removed from...Ch. 14 - Successful DNA synthesis requires all of the...Ch. 14 - The synthesis of telomeres a. uses DNA polymerase,...Ch. 14 - When mutations that affected DNA replication were...Ch. 14 - Prob. 1SCh. 14 - In the Meselson-Stahl experiment, a control...Ch. 14 - Enzyme function is critically important for the...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Avery, MacLeod, and McCarty demonstrated that the transforming principle can be destroyed by DNase. Thus, DNA is the genetic material A. Both statements are correct B. Both statements are incorrect C. First statement is Correct, Second is Incorrect D. First statement is Incorrect, Second is Correctarrow_forwardThe in situ hybridization method is based on Select one: a. labeling specific neuronal components with antibodies b. injecting a fluorescent dye into a neuron c. nucleic acid chains complementary to mRNAs that encode specific genes d. using nucleic acid probes to detect specific proteins e. formation of an insoluble colored product within cell bodiescarrow_forwardThe ab initio approach finds genes by looking for a. common sequences found in most genes. b. similarity in sequence with known genes. c. mRNA with the use of in situ hybridization. d. mutant phenotypesarrow_forward
- Which of the following is NOT used in an in situ hybridization experiment for visualizing DNA? (CSLO 1, CSLO 3, CSLO 4, CSLO 7) O A. chromosome spread O B. probe O C. denaturation solution D. antibody E. microscopearrow_forwardWhen E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as : A. Ligation. B. Transformation. C. Transfection. D. Digestion.arrow_forwardWhich of the following is true about DNA manipulation? Select the best answer. A. all of these B. DNA has never been transferred between different organisms. C. Transferring DNA from one organism into another always kills the host organism. D. Whole genomes have been manipulated by humans for centuries; now individual genes ca be inserted into different organisms using a variety of techniques. E. DNA can't be manipulated.arrow_forward
- Which one of the following statements is true? a. Dideoxynucleotides signal the end of DNA replication in a cell b. None of the provided answers are true c. The DNA sequence determined by an autoradiogram should be identical to the template strand DNA sequence d. The probe hybridization solution used in the Sothern blot technique is approximately pH 10.0 e. The transfer buffer used in the Southern blot technique is approximately pH 7.0arrow_forwardA.) Chemical changes to the DNA caused by mutations can cause hazardous damage to the DNA. B.) Environmental factors such as radiation can cause damage to the DNA of the cells. a. Statement A is correct b. Statement B is correct c. Both A and B are correct d. Both A and B are incorrectarrow_forwardWhat is Sanger sequencing? Why do we use ddNTP? How to read a DNA sequence gel? c. What is a cDNA seq (RNA seq)? d. What is the main difference between a genomic and a transcriptome study?arrow_forward
- For each situation, write the letter of the technique that would be most helpful; A. DNA editing A doctor wants to know if a patient has an inherited using CRISPR B. DNA replication using PCR C. DNA analysis through genetic testing D. DNA insertion 16. disorder. I A scientist needs many copies of a gene to conduct an 17. experiment. A genetic engineer wants to replace a defective copy of a gene with a functional copy in a chromosome. 18. into bacteria as a plasmid A medical researcher needs many copies of a protein 19. (insulin) to be produced to use in a medical treatment. A researcher crossed two purebred shrubs of the same species. One produces a fruit with a thin skin, and one produces a fruit with a thick skin. All of the plants resulting from the cross produce fruits with thick skins. Enter one letter in each blanks (19 & 20) to correctly complete the sentences. ninate Education TM, Inc.arrow_forwardWrite the advantages and disadvantages of applying such application in the DNA of an organism. a. Drug delivery systems that are based on bacterial or viral host. b. Producing genetically modified organisms to enhance food production. c. Combining two or more genes in one organism to enhance its traits. d. DNA manipulation of certain species to produce organs for harvesting.arrow_forwardWhich of the following experimental results was NOT evidence that DNA is the genetic molecule rather than proteins? a. When a virus was radioactively labelled, and the virus was allowed to infect a bacteria cell, radioactive virus DNA was found inside the bacteria cell while radioactive virus proteins were found outside the cell. b. Dead pathogenic bacteria cells combined with living nonpathogenic bacteria cells caused the creation of living pathogenic cells and thus the death of the host animal. c. Proteins are a class of macromolecules with the diversity and specificity needed for hereditary material. d. Nucleotide bases occur regularly, such that the number of adenines = number of thymines, and the number of guanines = number of cytosines.arrow_forward
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