Biology
Biology
12th Edition
ISBN: 9781260494570
Author: Raven, Peter
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 14, Problem 2DA
Summary Introduction

To determine: The products of a third round of replication in Meselson and Stahl’s experiment.

Introduction: Meselson and Stahl were the first to propose the semi-conservative mode of replication of DNA. They proved this replication feature by observing the pattern of replication in E. coli cells.

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What is meant by the semiconservative nature of DNA replication?
Suppose that replication is initiated in a medium containing moderately radioactive tritiated thymine. After a few minutes of incubation, the bacteria are transferred to a medium containing highly radioactive tritiated thymidine. Sketch the autoradiographic pattern that would be seen for (a) undirectional replication and (b) bidirectional replication, each from a single origin.
Where does  is the origin of replication?

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