FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
5th Edition
ISBN: 9781119661450
Author: Voet
Publisher: WILEY
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Chapter 14, Problem 28CQ
Summary Introduction
To calculate: The concentration of AMP.
Concept introduction: The equilibrium constant represents the relation between reactants and products in a reaction at equilibrium. The concentrations of reactants and products are constant at equilibrium. The spontaneity of a reaction depends on the decrease in free energy (ΔG), that is, free energy should be negative.
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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 14 Solutions
FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
Ch. 14 - Prob. 1ECh. 14 - 2. Methanogens are prokaryotes that produce...Ch. 14 - Prob. 3ECh. 14 - Prob. 4ECh. 14 - Prob. 5ECh. 14 - 6. In the partial reactions shown below, is the...Ch. 14 - Prob. 7ECh. 14 - 8. Choose the best definition for a near...Ch. 14 - Prob. 9ECh. 14 - 10. Nearly all enzymes that require a nicotinamide...
Ch. 14 - 11. Assuming 100% efficiency of energy...Ch. 14 - 12. Assuming 100% efficiency of energy...Ch. 14 - Prob. 13ECh. 14 - 14. The reaction catalyzed by malate...Ch. 14 - TABLE 14-4 Standard Reduction Potentials of Some...Ch. 14 - Prob. 16ECh. 14 - 17. Under standard conditions, will the following...Ch. 14 - 18. Under standard conditions, will the following...Ch. 14 - Prob. 19ECh. 14 - 20. Why do DNA chips often contain segments...Ch. 14 - Prob. 21ECh. 14 - Prob. 22ECh. 14 - 23. A certain metabolic reaction takes the form A...Ch. 14 - 24. Cells carry out anabolic as well as catabolic...Ch. 14 - Prob. 25CQCh. 14 - Prob. 26CQCh. 14 - Prob. 27CQCh. 14 - Prob. 28CQCh. 14 - Prob. 29CQCh. 14 - 30. The thioester described in Problem 29 reacts...Ch. 14 - Prob. 31CQCh. 14 - 32. Aerobic organisms transfer electrons from...Ch. 14 - Prob. 33CQCh. 14 - Prob. 34CQCh. 14 - 35. A hypothetical three-step metabolic pathway...Ch. 14 - 36. A certain metabolic pathway can be diagrammed...Ch. 14 - Prob. 1MTE
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