(a) Interpretation: The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined. Concept introduction: The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

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Answer 1

Question

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

Question

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

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Answer 3

Question

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

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Answer 4

Question

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

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Answer 5

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 6

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 7

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 10

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 11

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 12

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Answer 13

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Answer 14

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Answer 15

Ch. 14 - Prob. 1P Ch. 14 - Prob. 2P Ch. 14 - Prob. 3P Ch. 14 - Prob. 4P Ch. 14 - Prob. 5P Ch. 14 - Prob. 6P Ch. 14 - Prob. 7P Ch. 14 - Prob. 8P Ch. 14 - Prob. 9P Ch. 14 - Prob. 10P

Solution Summary: The author explains that the ligand binding assay is an analytic procedure, in which the receptor binding is the basis of the experiment.

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Chapter 14 Solutions