
(a)
To determine: The effect on the net reaction catalyzed by glyceraldehyde 3-phosphate dehydrogenase if phosphate were replaced by arsenate.
Introduction:
Arsenate (As) is chemically and structurally similar to inorganic phosphate and many other enzymes that need phosphate will also use arsenate. Organic compounds of arsenate are less stable compared to analogous phosphate compounds. On the other hand 1, 3-bisphosphoglycerate (acyl phosphates) is most stable and undergoes enzyme-catalyzed transformation in cells.
(b1)
To determine: The consequence to an organism if arsenate was substituted for phosphate.
Introduction:
Arsenate is chemically and structurally similar to inorganic phosphate and many other enzymes that need phosphate will also use arsenate. Organic compounds of arsenate are less stable compared to analogous phosphate compounds.
(b2)
To explain: The reason that arsenate is very toxic to most organisms.
Introduction:
Arsenate is chemically and structurally similar to inorganic phosphate and many other enzymes that need phosphate will also use arsenate. Organic compounds of arsenate are less stable compared to analogous phosphate compounds.

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Chapter 14 Solutions
Loose-leaf Version for Lehninger Principles of Biochemistry 7E & SaplingPlus for Lehninger Principles of Biochemistry 7E (Six-Month Access)
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