GENETICS(LL)-W/CONNECT >CUSTOM<
GENETICS(LL)-W/CONNECT >CUSTOM<
6th Edition
ISBN: 9781260571561
Author: HARTWELL
Publisher: MCG CUSTOM
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Chapter 14, Problem 14P

In two isolates (one is resistant to ampicillin, and the other is sensitive to ampicillin) of a new bacterium, you

found that genes encoding ampicillin resistance are being transferred into the sensitive strain

a. How would you know that gene transfer is taking place?
b. To determine if the gene transfer is transformation or transduction, you treat the mixed culture of cells with DNase. Why would this treatment distinguish between these two modes of gene transfer? Describe the results predicted if the gene transfer is transformation versus transduction.
c. To determine if the gene transfer involves transformation, conjugation, or transduction, you separate the ampicillin-resistant and ampicillin-sensitive strains by a membrane with pores that are smaller than the size of a bacterium, but larger than the sizes of bacteriophage or DNA fragments. If gene transfer is still observed, what mechanisms are possibly involved, and which are excluded?
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Respond to the following in a minimum of 175 words:  How far might science go in the name of genetic research, and at what point should genetic research be regulated? What types of ethical issues might arise from altering the genes of humans, animals, and plants? Provide an example and discuss the advantages and disadvantages of genetic experimentation.
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