GENETICS(LL)-W/CONNECT >CUSTOM<
6th Edition
ISBN: 9781260571561
Author: HARTWELL
Publisher: MCG CUSTOM
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Chapter 14, Problem 14P
In two isolates (one is resistant to ampicillin, and the other is sensitive to ampicillin) of a new bacterium, you
found that genes encoding ampicillin resistance are being transferred into the sensitive strain
| a. | How would you know that gene transfer is taking place? |
| b. | To determine if the gene transfer is transformation or transduction, you treat the mixed culture of cells with DNase. Why would this treatment distinguish between these two modes of gene transfer? Describe the results predicted if the gene transfer is transformation versus transduction. |
| c. | To determine if the gene transfer involves transformation, conjugation, or transduction, you separate the ampicillin-resistant and ampicillin-sensitive strains by a membrane with pores that are smaller than the size of a bacterium, but larger than the sizes of bacteriophage or DNA fragments. If gene transfer is still observed, what mechanisms are possibly involved, and which are excluded? |
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Chapter 14 Solutions
GENETICS(LL)-W/CONNECT >CUSTOM<
Ch. 14 - Choose the phrase from the right column that best...Ch. 14 - The unicellular, rod-shaped bacterium E. coli is 2...Ch. 14 - Now that the sequence of the entire E. coli K12...Ch. 14 - Bacterial genomes such as that of E. coli...Ch. 14 - List at least three features of eukaryotic genomes...Ch. 14 - Describe a mechanism by which a gene could move...Ch. 14 - High salt concentrations tend to cause protein...Ch. 14 - Recently, scientists tested the possibility that...Ch. 14 - A recent metagenomic study analyzed the...Ch. 14 - Linezolid is a new type of antibiotic that...
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