GENETICS(LL)-W/CONNECT >CUSTOM<
GENETICS(LL)-W/CONNECT >CUSTOM<
6th Edition
ISBN: 9781260571561
Author: HARTWELL
Publisher: MCG CUSTOM
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Chapter 14, Problem 19P
a. You want to perform an interrupted-mating mapping with an E. coli Hfr strain that is Pyr, Met, Xyl
Tyr, Arg, His, Mal, and Strs. Describe an appropriate bacterial strain to be used as the other partner in this mating.
b. In an Hfr × F cross, the pyrE gene enters the recipient in 5 minutes, but at this time point there are no exconjugants that are Met, Xyl, Tyr, Arg, His, or Mal . The mating is now allowed to proceed for 30 minutes and Pyr exconjugants are selected. Of the Pyr cells, 32% are Met, 94% are Xyl, 7% are Tyr, 59% are Arg, 0% are His, and 71% are Mal . What can you conclude about the order of the genes?
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