The advantages and disadvantages of aluminium alloys that can replace structural steels in ships needs to be explained. Concept Introduction: Aluminum ranks the third most abundantat available component on the planet but, until the year 1800, it was costly. Aluminum can be molded effortlessly and it has high thermal and electricalconductivitie properties and at low temperatures it does not show a ductile to brittle changeover. Approximately 25% of aluminum formed currently is applicable in the transportation business, another 25% in the production of beverages cans and packaging, 15% in building, 15% in electrical applications and 20 % in other applications.

Question

Bioprocessing/ Protein isolation and purification. Develop a purification train for a facility where first process is a perfusion upstream bioreactors 500L producing low cell culture titer of approx. 0.5 g/L perfusing at 2 VVD over 30 days. The current facility has a secondary clarification process for the perfusate coming from the bioreactor. Secondary depth filtration clarification capacity of 200 L/m2. Identify the correct filter area, and system (pump) requirements for the process scale. Also identify optimal flowrates for flushing and processing, total process time, buffer volumes required. Assume 10 L/m2 holdup of the depth filters identify the size of the tank required to collect the filtrate. Average yield of overall clarification is 80% estimate the titer in the clarified pool.

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Question

Chapter 14, Problem 14.4P

Interpretation Introduction

Interpretation:

The advantages and disadvantages of aluminium alloys that can replace structural steels in ships needs to be explained.

Concept Introduction:

Aluminum ranks the third most abundantat available component on the planet but, until the year 1800, it was costly. Aluminum can be molded effortlessly and it has high thermal and electricalconductivitie properties and at low temperatures it does not show a ductile to brittle changeover. Approximately 25% of aluminum formed currently is applicable in the transportation business, another 25% in the production of beverages cans and packaging, 15% in building, 15% in electrical applications and 20 % in other applications.

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Bioprocessing/ Protein isolation and purification. Develop a purification train for a facility where first process is a perfusion upstream bioreactors 500L producing low cell culture titer of approx. 0.5 g/L perfusing at 2 VVD over 30 days. The current facility has a secondary clarification process for the perfusate coming from the bioreactor. Secondary depth filtration clarification capacity of 200 L/m2. Identify the correct filter area, and system (pump) requirements for the process scale. Also identify optimal flowrates for flushing and processing, total process time, buffer volumes required. Assume 10 L/m2 holdup of the depth filters identify the size of the tank required to collect the filtrate. Average yield of overall clarification is 80% estimate the titer in the clarified pool.

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