Foundations in Microbiology
10th Edition
ISBN: 9781259705212
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Chapter 13.4, Problem 27CYP
What is epidemiologically and medically important about carriers in the population?
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 13 Solutions
Foundations in Microbiology
Ch. 13.1 - Describe some of the major interactions between...Ch. 13.1 - Prob. 2ELOCh. 13.1 - Discuss the characteristics of the normal...Ch. 13.1 - Briefly relate the sources and conditions that...Ch. 13.1 - Identify which bodily sites remain free of living...Ch. 13.1 - Prob. 6ELOCh. 13.1 - Prob. 1CYPCh. 13.1 - Prob. 2CYPCh. 13.1 - Prob. 3CYPCh. 13.1 - Prob. 4CYP
Ch. 13.1 - Prob. 5CYPCh. 13.1 - Differentiate between transient and resident...Ch. 13.1 - Explain the factors that cause variations in the...Ch. 13.2 - Review the main stages in the development of an...Ch. 13.2 - Prob. 8ELOCh. 13.2 - Prob. 9ELOCh. 13.2 - Prob. 10ELOCh. 13.2 - Prob. 11ELOCh. 13.2 - Identify and discuss invasive factors and...Ch. 13.2 - Prob. 13ELOCh. 13.2 - Explain several ways in which true pathogens...Ch. 13.2 - Distinguish between pathogenicity and virulence.Ch. 13.2 - Prob. 10CYPCh. 13.2 - Prob. 11CYPCh. 13.2 - Prob. 12CYPCh. 13.2 - Describe several components of pathogens that are...Ch. 13.2 - Prob. 14CYPCh. 13.2 - Prob. 15CYPCh. 13.2 - Define toxigenicity and summarize the main...Ch. 13.2 - Prob. 17CYPCh. 13.3 - Describe the clinical stages of infection.Ch. 13.3 - Use key terms to describe different patterns of...Ch. 13.3 - Prob. 16ELOCh. 13.3 - Prob. 17ELOCh. 13.3 - Explain what is happening during each stage of...Ch. 13.3 - Prob. 19CYPCh. 13.3 - Name some examples of infections and their portals...Ch. 13.3 - 21. Using terminology from this section's “Guide...Ch. 13.4 - Define epidemiology, and summarize the major goals...Ch. 13.4 - Prob. 19ELOCh. 13.4 - Prob. 20ELOCh. 13.4 - Prob. 21ELOCh. 13.4 - Prob. 22ELOCh. 13.4 - Prob. 23ELOCh. 13.4 - Prob. 22CYPCh. 13.4 - Prob. 23CYPCh. 13.4 - Prob. 24CYPCh. 13.4 - Prob. 25CYPCh. 13.4 - Prob. 26CYPCh. 13.4 - What is epidemiologically and medically important...Ch. 13.5 - Prob. 24ELOCh. 13.5 - Prob. 25ELOCh. 13.5 - Summarize the steps in Koch’s postulates, and...Ch. 13.5 - Prob. 27ELOCh. 13.5 - Prob. 28ELOCh. 13.5 - Prob. 28CYPCh. 13.5 - Prob. 29CYPCh. 13.5 - Prob. 30CYPCh. 13.5 - Prob. 31CYPCh. 13.5 - Prob. 32CYPCh. 13.5 - Outline the major factors involved in...Ch. 13.L1 - Prob. 1MCQCh. 13.L1 - Prob. 2MCQCh. 13.L1 - Prob. 3MCQCh. 13.L1 - Prob. 4MCQCh. 13.L1 - Prob. 5MCQCh. 13.L1 - Prob. 6MCQCh. 13.L1 - Prob. 7MCQCh. 13.L1 - The presence of a few bacteria in the blood is...Ch. 13.L1 - Prob. 9MCQCh. 13.L1 - A/an ______ is a passive animal transporter of...Ch. 13.L1 - Prob. 11MCQCh. 13.L1 - Prob. 12MCQCh. 13.L1 - Prob. 13MCQCh. 13.L1 - A positive antibody test for HIV would be a...Ch. 13.L1 - Prob. 15MCQCh. 13.L1 - Prob. 16MCQCh. 13.L1 - Prob. 1CSRCh. 13.L1 - Prob. 2CSRCh. 13.L1 - Prob. 3CSRCh. 13.L1 - Prob. 1WCCh. 13.L1 - Prob. 2WCCh. 13.L1 - Prob. 3WCCh. 13.L1 - Prob. 4WCCh. 13.L1 - Prob. 5WCCh. 13.L1 - Prob. 6WCCh. 13.L1 - Prob. 7WCCh. 13.L1 - a. Outline the five types of clinical isolation....Ch. 13.L1 - Complete the following table. Chemical makeup...Ch. 13.L2 - Discuss the relationship between the vaginal...Ch. 13.L2 - Prob. 2CTCh. 13.L2 - How could the microbiome cause some infections to...Ch. 13.L2 - Each of the nine patient specimens listed below...Ch. 13.L2 - Prob. 5CTCh. 13.L2 - Prob. 6CTCh. 13.L2 - Prob. 7CTCh. 13.L2 - a. Suggest several reasons why respiratory,...Ch. 13.L2 - Summarize the epidemiological findings in the...Ch. 13.L2 - Looking at figure 13.20b. Which pattern of...Ch. 13.L2 - Prob. 1VCCh. 13.L2 - Observe the following maps (a)-(c) of three...Ch. 13.L2 - Prob. 3VC
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have shipped your samples off for sequencing and are now waiting for the results. Out of curiosity (and maybe boredom...) you decide to test your culture for the Catalase and Oxidase enzymes. Upon testing your sample for catalase, you don't see any bubbles; however, you do see a color change to purple during the Oxidase test. What results can you conclude from this? O Catalase-/ Oxidase + O Catalase +/ Oxidase + Catalase + / Oxidase- O Catalase / Oxidase - O None of the abovearrow_forwardWhich of the following is not a strength of using 16S rRNA for phylogenetic analyses? OA. It's cheap OB. It's easy to do C. It can be used to identify all the way down to the strain level OD. Both A & B OE. None of the abovearrow_forwardWhy are molecular approaches important to the field of microbial taxonomy and phylogeny? Phylogenetic inferences based on molecular approaches provide the most robust analysis of microbial evolution currently available. It allows for the collection of a large and accurate dataset from many organisms Almost no fossil record was left by microbes when compared to plants and animals All of the above None of the abovearrow_forward
- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
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