The results that would be expected in the experiment given in “Figure 12.17 (Experimental procedure for studying how nucleosomes dissociate and reassociate in the course of replication)”, as if all the original histone proteins remained on one strand of the DNA and new histone attached to the other strand. Introduction: If histone protein makes complexes with eukaryotic DNA, it leads to form nucleosome assembly. Nucleosomes are contributing to the stability and packaging of DNA. During replication, the structure of histone protein disrupted by the replication fork, however, in a short time duration nucleosomes reassembled on the newly synthesized strands of DNA.

Question

Transcription and Translation 1. What is the main function of transcription and translation? (2 marks) 2. How is transcription different in eukaryotic and prokaryotic cells? (2 marks) 3. Explain the difference between pre-mRNA and post-transcript mRNA. (2 marks) 4. What is the function of the following: (4 marks) i. the cap ii. spliceosome iii. Poly A tail iv. termination sequence 5. What are advantages to the wobble feature of the genetic code? (2 marks) 6. Explain the difference between the: (3 marks) i. A site & P site ii. codon & anticodon iii. gene expression and gene regulation 7. Explain how the stop codon allows for termination. (1 mark) 8. In your own words, summarize the process of translation. (2 marks)

Answer 1

Question

Chapter 12.4, Problem 32AQP

Summary Introduction

To determine:

The results that would be expected in the experiment given in “Figure 12.17 (Experimental procedure for studying how nucleosomes dissociate and reassociate in the course of replication)”, as if all the original histone proteins remained on one strand of the DNA and new histone attached to the other strand.

Introduction:

If histone protein makes complexes with eukaryotic DNA, it leads to form nucleosome assembly. Nucleosomes are contributing to the stability and packaging of DNA. During replication, the structure of histone protein disrupted by the replication fork, however, in a short time duration nucleosomes reassembled on the newly synthesized strands of DNA.

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