Toexplain: The reason whyboth proofreading and priming functions are incompatible in the DNA polymerase. Introduction: The DNA polymerase enzyme uses a single-stranded DNA molecule as a template and causes the synthesis of another strand. DNA polymerases play important roles for replication of DNA and formation of two identical strands of DNA from one parent strand. DNA polymerases can “read” the parent strand to make new strands which are complementary to the parent strand. Primase is an RNA polymerase that synthesizes short RNA sequences called “primers”. Primers are complementary strands of a single-stranded DNA (ssDNA) template. Primers incorporate dNTPs into the template on the 3 ′ OH group that can attach to the incoming dNTPs. Primers are required by DNA polymerase to add DNA nucleotides .

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Chapter 12.3, Problem 3TPSQ

Summary Introduction

Toexplain:

The reason whyboth proofreading and priming functions are incompatible in the DNA polymerase.

Introduction:

The DNA polymerase enzyme uses a single-stranded DNA molecule as a template and causes the synthesis of another strand. DNA polymerases play important roles for replication of DNA and formation of two identical strands of DNA from one parent strand. DNA polymerases can “read” the parent strand to make new strands which are complementary to the parent strand.

Primase is an RNA polymerase that synthesizes short RNA sequences called “primers”. Primers are complementary strands of a single-stranded DNA (ssDNA) template. Primers incorporate dNTPs into the template on the 3′ OH group that can attach to the incoming dNTPs. Primers are required by DNA polymerase to add DNA nucleotides.

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