Genetics: A Conceptual Approach 6E w/ SaplingPlus (Six-Month Access)
Genetics: A Conceptual Approach 6E w/ SaplingPlus (Six-Month Access)
6th Edition
ISBN: 9781319125929
Author: Benjamin A. Pierce
Publisher: W. H. Freeman
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Chapter 12.3, Problem 30AQP
Summary Introduction

To determine:

The effect of mutation on the gene of primase on theta replication.

Introduction:

Primase is a RNA polymerase that synthesizes short RNA sequences called “primers”. Primers are complementary strands of a single stranded DNA (ssDNA) template. Primers incorporate dNTPs on the template on the 3 OH group that can attach to the incoming dNTPs. Primers are required by DNA polymerase to add DNA nucleotides.

Summary Introduction

To determine:

The effect of mutation on the gene of primase on rolling circle replication.

Introduction:

The rolling circle method of DNA replicationdemonstrates a unidirectional replication of nucleic acid which synthesizes multiple copies of DNA or RNA rapidly. For example, plasmids, bacteriophages, and the genome of virioids. In this replication,“the outer strand works as a positive strand and the inner strand works as negative strand”. DNA replication begins to start by creating a nick (cut) at positive strand. The strand has served as a template for the strand. The negative strand serves as a template for making the  strand.

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*Example 2: Tracing the path of an autosomal dominant trait Trait: Neurofibromatosis Forms of the trait: The dominant form is neurofibromatosis, caused by the production of an abnormal form of the protein neurofibromin. Affected individuals show spots of abnormal skin pigmentation and non-cancerous tumors that can interfere with the nervous system and cause blindness. Some tumors can convert to a cancerous form. i The recessive form is a normal protein - in other words, no neurofibromatosis.moovi A typical pedigree for a family that carries neurofibromatosis is shown below. Note that carriers are not indicated with half-colored shapes in this chart. Use the letter "N" to indicate the dominant neurofibromatosis allele, and the letter "n" for the normal allele. Nn nn nn 2 nn Nn A 3 N-
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