Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Textbook Question
Chapter 11.9, Problem 1CR
Q What is a merodiploid and how does an F′ plasmid yield a merodiploid?
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Chapter 11 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 11.1 - Distinguish between a mutation and a mutant.Ch. 11.1 - Distinguish between screening and selection.Ch. 11.1 - Prob. 3MQCh. 11.1 - Write a one-sentence definition of the term...Ch. 11.2 - Do missense mutations occur in genes encoding...Ch. 11.2 - Why do frameshift mutations generally have more...Ch. 11.2 - Prob. 1CRCh. 11.3 - Why are suppressor tRNA mutations not lethal?Ch. 11.3 - Which class of mutation, missense or nonsense, is...Ch. 11.3 - What is the difference between same-site and...
Ch. 11.4 - Prob. 1MQCh. 11.4 - Prob. 2MQCh. 11.4 - Prob. 1CRCh. 11.5 - Which protein, found in virtually all cells,...Ch. 11.5 - Explain the fate of transferred chromosomal DNA if...Ch. 11.5 - Prob. 3MQCh. 11.5 - What are heteroduplex regions of DNA and what...Ch. 11.6 - During transformation a cell usually incorporates...Ch. 11.6 - In genetic transformation, what is meant by the...Ch. 11.6 - QExplain why recipient cells do not successfully...Ch. 11.7 - Prob. 1MQCh. 11.7 - What is the major difference between generalized...Ch. 11.7 - Why is phage conversion considered beneficial to...Ch. 11.7 - QExplain how a generalized transducing particle...Ch. 11.8 - In conjugation, how are donor and recipient cells...Ch. 11.8 - Explain how rolling circle DNA replication allows...Ch. 11.8 - QWhat is a sex pilus and which cell type, F or F+,...Ch. 11.9 - In conjugation involving the F plasmid of...Ch. 11.9 - Prob. 2MQCh. 11.9 - Prob. 3MQCh. 11.9 - QWhat is a merodiploid and how does an F plasmid...Ch. 11.10 - Why is it usually more difficult to select...Ch. 11.10 - Why do penicillins not kill species of Archaea?Ch. 11.10 - Explain one type of conjugation in Archaea and how...Ch. 11.11 - Prob. 1MQCh. 11.11 - What is the significance of the terminal inverted...Ch. 11.11 - How can transposons be used in bacterial genetics?Ch. 11.11 - Prob. 1CRCh. 11.12 - Why is the CRISPR system considered a prokaryotic...Ch. 11.12 - Prob. 2MQCh. 11.12 - QExplain why incoming DNA recognized by a short...Ch. 11 - A constitutive mutant is a strain that...Ch. 11 - Although a large number of mutagenic chemicals are...Ch. 11 - Why is it difficult in a single experiment to...Ch. 11 - Prob. 4AQ
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- 1 (a) What do ApR, TcR, and ori on the pBR322 map represent and discuss there individual functions? (b)Does the undigested plasmid show more than a single band when electrophoresed? Why? (c)What other kinds of molecules, in addition to plasmid DNA would you expect to find in a sample of plasmid DNA extraction? (This is not a graded assignment)arrow_forwardWhat is the role of X-gal? What color will cells with the plasmid be in the absence of X-gal? Why?arrow_forwardWhat is true regarding the following cross between bacterial strains: F’ x F-? As the donor, F' will donate only the F plasmid to F- As the donor, F- will donate the F plasmid only to F' As the donor, F- will donate the F plasmid and a portion of the bacterial chromosome to F' As the donor, F' will donate the F plasmid and a portion of the bacterial chromosome to F-arrow_forward
- In the genotype presented (genomic plus plasmid genes), which of the following statements Is phenotype? genome: IS P O`z+Y° plasmid: I* Pt otzY+ no production of either B-gal and permease O B-gal and permease induced when lactose is present no B-gal is produced and expression of permease when lactose is present constitutive expression of B-gal and permease constitutive expression of B-gal and no expression of permeasearrow_forwardCalculate the linking number “L” for a 210 base pair closed circular plasmid with 4 negative supercoils. Assume the DNA remains entirely B-form. A) 10 B) 16 C) 24 D) -10 E) -4 Please solve and why is it B? Need answer in short and asap .arrow_forwardAssume a 5250 base pair, closed circular plasmid with 10 negativesupercoils.(a) Calculate the values of twist (T), writhe (W), and linking number (L) for thisplasmid (use 10.5 base pairs/turn for B-DNA)(b) Your starting plasmid is now A-DNA instead of B-DNA. What is the linkingnumber required to have 10 negative supercoils in this plasmid? (R = 8.3145 J−1 K mol−1; 0 ◦C = 273.15 K; Faraday Constant , F =96485 J V−1 mol−1.)arrow_forward
- What is a merodiploid and how does an F¿ plasmidyield a merodiploid?arrow_forwardHow is telomerases related to ageing? How Can telomerase- related ageing be addressed therapeutically?arrow_forwardWhat happens to YAC • Plasmids containing only origins of replication but no centromere or telomere?arrow_forward
- What is the direction of transcription in the plasmid shown below? Explain your answer based on the features present in the figure.arrow_forwardWhat is the relationship between a PRR and a PAMP?arrow_forward1)What is plasmid? 2) How do you explain the selection of plasmid for carrying the desired gene? 3) Follow the steps of human insulin production 4) Make a diagram for the production of growth hormonearrow_forward
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