Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Textbook Question
Chapter 11.7, Problem 3MQ
- Why is phage conversion considered beneficial to host cells?
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Why does phage T4 need a lysozyme-like protein in order toinfect its host, and what part of T4 enters the host cytoplasm?
Describe the reason behind the occurrence of phage growth and release by the mating with the non-lysogenic recipient, but not with the infection by a lysogenic recipient.
phage can be "induced" as a result of DNA damage. Consequences of
induction include.
Temperate phage, e.g lambda phage; lytic cycle and transduction
Lytic T4 phage, assembly, conjugation
O Lytic phage, e.g. T4 phage; lysogenic cycle, prophage formation
Temperate phage, e.g. lambada phage, prophage formation, transformation
O T4 phage, lytic cycle, specialized trasnduction
Chapter 11 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 11.1 - Distinguish between a mutation and a mutant.Ch. 11.1 - Distinguish between screening and selection.Ch. 11.1 - Prob. 3MQCh. 11.1 - Write a one-sentence definition of the term...Ch. 11.2 - Do missense mutations occur in genes encoding...Ch. 11.2 - Why do frameshift mutations generally have more...Ch. 11.2 - Prob. 1CRCh. 11.3 - Why are suppressor tRNA mutations not lethal?Ch. 11.3 - Which class of mutation, missense or nonsense, is...Ch. 11.3 - What is the difference between same-site and...
Ch. 11.4 - Prob. 1MQCh. 11.4 - Prob. 2MQCh. 11.4 - Prob. 1CRCh. 11.5 - Which protein, found in virtually all cells,...Ch. 11.5 - Explain the fate of transferred chromosomal DNA if...Ch. 11.5 - Prob. 3MQCh. 11.5 - What are heteroduplex regions of DNA and what...Ch. 11.6 - During transformation a cell usually incorporates...Ch. 11.6 - In genetic transformation, what is meant by the...Ch. 11.6 - QExplain why recipient cells do not successfully...Ch. 11.7 - Prob. 1MQCh. 11.7 - What is the major difference between generalized...Ch. 11.7 - Why is phage conversion considered beneficial to...Ch. 11.7 - QExplain how a generalized transducing particle...Ch. 11.8 - In conjugation, how are donor and recipient cells...Ch. 11.8 - Explain how rolling circle DNA replication allows...Ch. 11.8 - QWhat is a sex pilus and which cell type, F or F+,...Ch. 11.9 - In conjugation involving the F plasmid of...Ch. 11.9 - Prob. 2MQCh. 11.9 - Prob. 3MQCh. 11.9 - QWhat is a merodiploid and how does an F plasmid...Ch. 11.10 - Why is it usually more difficult to select...Ch. 11.10 - Why do penicillins not kill species of Archaea?Ch. 11.10 - Explain one type of conjugation in Archaea and how...Ch. 11.11 - Prob. 1MQCh. 11.11 - What is the significance of the terminal inverted...Ch. 11.11 - How can transposons be used in bacterial genetics?Ch. 11.11 - Prob. 1CRCh. 11.12 - Why is the CRISPR system considered a prokaryotic...Ch. 11.12 - Prob. 2MQCh. 11.12 - QExplain why incoming DNA recognized by a short...Ch. 11 - A constitutive mutant is a strain that...Ch. 11 - Although a large number of mutagenic chemicals are...Ch. 11 - Why is it difficult in a single experiment to...Ch. 11 - Prob. 4AQ
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- Bacteriophage T4 lacks its own RNA polymerase.How do T4 genes get expressed or converted to mRNA?What host barriers must be broken before release ofvirions from the host cell?arrow_forwardWhat is the toxin produced by E. coli O157:H7 that was encode by phage as a consequence of lysogenic conversion? Explain the medical importance.arrow_forwardWhat is required to package the T4 genome into its phage head?arrow_forward
- What is the difference between the lytic and lysogenic cycle of bacteriophages? In the lysogenic cycle, the phage DNA integrates itself into the host genome after penetration, and continues to biosynthesis when conditions change The host cell is not killed in the lysogenic cycle The lytic cycle occurs in the nucleus, while the lysogenic cycle occurs in the cytoplasm In the lysogenic cycle, penetration is achieved through phagocytosisarrow_forwardWhy is a webbed plate capable of producing a higher lysate titer (pfu/mL) than a cleared plate? a.) A webbed plate has plaques that have gone through more rounds of replication because the number of host cells did not limit phage replication, creating exponentially more phage particles b.) A webbed plate has no more host available for replication, meaning the plate is full of exponentially higher amounts of phage c.) A cleared plate has less bacteria than a webbed plate, so there are more plaques present d.) A cleared plate contains more phage than a webbed plate because it started with a more concentrated phage samplearrow_forwardThis gene is responsible for making the protein that catalyzes the recombination of the phage genome into the host bacterial chromosome is: excise integrase attP attBarrow_forward
- You are studying your favorite (hypothetical) bacteriophage named M277. It infects E. coli and stays dormant within the bacterial cell. How does a phage become dormant within a cell? Generally, describe the steps of infection by phage in this scenarioarrow_forwardWhat is wrongly matched? A) None of the above B) Transfer of genetic material from 1 cell to --- conjugation. Another involving cell to cell contact C) Transfer of DNA from 1 cell to another by a Bacteriophage---Transduction D) Bacterial cell in which the F factor has become integrated---HFrarrow_forwardWhich of the statements is TRUE about conjugation? a) Plasmid DNA is transferred from a donor (F+) cell b) It may only be performed by competent cells c) DNA is always integrated into recipient chromosome d) It may be specialized or generalizedarrow_forward
- The mechanism of specialized transduction relies on the improper excision of a bacteriophage genome that is integrated in the host cell chromosom A) O True B) O Falsearrow_forwardSome mutations that occur in bacteria can cause the loss of phage receptors, and these bacteria become phage resistant. In order for a phage to infect the host bacterium, it is preferred that the cell wall is newly synthesized.why ?arrow_forwardWhy must the top agar be supplemented with MgSo4 A) it is required to induce the production of cro repressors B) it is required to induce the production of cI repressors C) it helps stabilize the phage for infection D) it induces the expression of the lamB gene in E. Coliarrow_forward
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