BIOLOGY
12th Edition
ISBN: 9781264839698
Author: Raven
Publisher: MCG CUSTOM
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Chapter 11.2, Problem 2LO
Summary Introduction
To describe: The reason that why meiosis I is called the reductive division.
Introduction: The reproduction is defined as the biological process in which the new individual organism or offspring are produced by their parents. It is divided into two types namely asexual reproduction and sexual reproduction. Most of the plants and animals reproduce sexually.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 11 Solutions
BIOLOGY
Ch. 11.1 - Prob. 1LOCh. 11.1 - Prob. 2LOCh. 11.2 - Describe how homologous chromosomes pair during...Ch. 11.2 - Prob. 2LOCh. 11.3 - Prob. 1LOCh. 11.3 - Prob. 2LOCh. 11.3 - Prob. 3LOCh. 11.4 - Prob. 1LOCh. 11.4 - Prob. 2LOCh. 11.4 - Explain the importance of the suppression of...
Ch. 11 - Prob. 1IQCh. 11 - In comparing somatic cells and gametes, somatic...Ch. 11 - What are homologous chromosomes? a. The two halves...Ch. 11 - Chiasmata form a. between homologous chromosomes....Ch. 11 - Crossing over involves each of the following with...Ch. 11 - During anaphase I a. sister chromatids separate...Ch. 11 - At metaphase I the kinetochores of sister...Ch. 11 - What occurs during anaphase of meiosis II? a. The...Ch. 11 - Which of the following does NOT contribute to...Ch. 11 - How does DNA replication differ between mitosis...Ch. 11 - Which of the following is NOT a distinct feature...Ch. 11 - Which phase of meiosis I is most similar to the...Ch. 11 - Structurally, meiotic cohesins have different...Ch. 11 - Prob. 6ACh. 11 - Diagram the process of meiosis for an imaginary...Ch. 11 - Mules are the offspring of the mating of a horse...Ch. 11 - Compare the processes of independent assortment...Ch. 11 - Aneuploid gametes are cells that contain the wrong...
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