EBK CONCEPTS OF GENETICS
12th Edition
ISBN: 9780134818979
Author: Killian
Publisher: YUZU
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Chapter 11, Problem 7PDQ
In Kornberg’s initial experiments, it was rumored that he grew E. coli in Anheuser-Busch beer vats. (Kornberg was working at Washington University in St. Louis.) Why do you think this might have been helpful to the experiment?
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a) What is the hypothesis of Griffith’s experiment?
b) Why did mouse No. 3 survived?
c) Why did mouse No. 4 died?
d) If Griffith had injected a fifth mouse with a combination of heat-killed R strain bacteria and heat-killed S strain bacteria, would the mouse have died? Give your reason.
All of the following apply to Luria and Delbruck’s 1943 study of mutation rates in E. Coli and T1 phage except:
A) it served as an example of an inflexible test
B) it involved looking for T1-resistant bacteria as an end measure
C) it showed that numbers of mutant organisms observed after T 1 phage were added to the culture tended to vary from experiment to experiment
D) it rejected the possibility of adaptive mutations
E) it supported the concept of spontaneous mutation rates
You got an opportunity to join a professor lab who is working in-vivo model and specifically looking at the dysregulation of mitochondria in liver. He asked you to isolate mitochondria from a Rat liver and placed in an assay medium. Based on the knowledge you gain in this course so far, please answer the following questions:
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Chapter 11 Solutions
EBK CONCEPTS OF GENETICS
Ch. 11 - In the Meselson-Stahl experiment, which of the...Ch. 11 - An alien organism was investigated. When DNA...Ch. 11 - Why might mutations in genes encoding telomerase...Ch. 11 - Although the brother is an immunologically matched...Ch. 11 - Prob. 3CSCh. 11 - HOW DOWE KNOW? In this chapter, we focused on how...Ch. 11 - CONCEPT QUESTION Review the Chapter Concepts list...Ch. 11 - Compare conservative, semiconservative, and...Ch. 11 - Describe the role of 15N in the MeselsonStahl...Ch. 11 - Predict the results of the experiment by Taylor,...
Ch. 11 - What are the requirements for in vitro synthesis...Ch. 11 - In Kornbergs initial experiments, it was rumored...Ch. 11 - How did Kornberg assess the fidelity of DNA...Ch. 11 - Which characteristics of DNA polymerase I raised...Ch. 11 - Kornberg showed that nucleotides are added to the...Ch. 11 - What was the significance of the polA1 mutation?Ch. 11 - Summarize and compare the properties of DNA...Ch. 11 - List and describe the function of the ten subunits...Ch. 11 - Distinguish between (a) unidirectional and...Ch. 11 - List the proteins that unwind DNA during in vivo...Ch. 11 - Define and indicate the significance of (a)...Ch. 11 - Outline the current model for DNA synthesis.Ch. 11 - Why is DNA synthesis expected to be more complex...Ch. 11 - Suppose that E. coli synthesizes DNA at a rate of...Ch. 11 - Several temperature-sensitive mutant strains of E....Ch. 11 - While many commonly used antibiotics interfere...Ch. 11 - Describe the end-replication problem in...Ch. 11 - Many of the gene products involved in DNA...Ch. 11 - In 1994, telomerase activity was discovered in...Ch. 11 - The genome of D. melanogaster consists of...Ch. 11 - Prob. 26ESPCh. 11 - DNA polymerases in all organisms add only 5...Ch. 11 - Assume that the sequence of bases shown below is...Ch. 11 - Reiji and Tuneko Okazaki conducted a now classic...Ch. 11 - Consider the drawing of a dinucleotide below. (a)...Ch. 11 - To gauge the fidelity of DNA synthesis, Arthur...
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- QUESTION 2 In the Luria Delbruck experiment, what did the appearance of a bacterial colony on a petri plate indicate? O A) The bacterial colony was derived from one initial cell that was resistant to infection by the bacteriophage. O B) The bacterial colony was made up of many cells that became resistant to bacteriophage infection independently. O C)A colony formed when a bacterial cell was not resistant to bacteriophage infection. O D) The bacterial cells that made up a colony were resistant to antibiotic. O E. phage caused mutations to occur in some of the plates but not in others.arrow_forwardChain-termination is a type of ______________a) Sequencingb) Vector generationc) Antibiotic productiond) Gene manipulationarrow_forwardThe question is: A patient has arrived at the doctor complaining of acute respiratory symptoms (cough, runny nose, fever). The patient explains to the doctor that he was at a concert the night before and shared a water bottle with a friend who had similar symptoms. The doctor tells the patient that he has a virus. a.) What form of replication do you think this virus does use? How do you know? b.) Can the doctor prescribe an antibiotic for this patient? Explain.arrow_forward
- Some bacteria may have higher mutation rates than others following exposure to UV light. Discuss a reason why this might be the case. What experiments could you do to determine whether this is a likely possibility?arrow_forwardWhich of the following statements best explains why there are fewer colonies on plate IV than on plate III? (a) Plate IV is the positive control. (b) Not all bacterial cells were successfully transformed. (c) The bacteria on plate III did not mutate. (d) The plasmid inhibited bacterial growth. (e) Plate IV is the negative control.arrow_forwardCytopathic effects (CPE) are visible changes in cells due to: A)viral infection B)mutations C)bacterial infections D)the cell's genes are being expressedarrow_forward
- Picture below shows the Hershey and Chase experiment. Identify where viral protein and viral DNA would be located by dragging the labels to the correct targets.arrow_forwardWhat else was unexpected in Griffith's experiment? a. )Griffith recovered infectios S train pneumonococci from the heart of the live mice b.)Griffith recovered infectios S train pneumonococci from the heart of the dead mice c.)Griffith recovered infectios R train pneumonococci from the heart of the live mice d.) Griffith recovered infectios R train pneumonococci from the heart of the live micearrow_forwardWhat do you mean by bacteriophage?arrow_forward
- a) Sam wants to alter his interrupted mating experiment by placing a filter in between the donor and the recipient cells in order to separate the two. Is Sam’s suggestion for this modification to the procedure utilized in interrupted mating experiments valid? Explain. b) Much to the dismay of the researcher with whom Sam is working, Sam is not only forgetful, but he is also lazy! Rather than devoting the necessary hours to conducting his assigned interrupted mating experiments, Sam has decided that his time would be better spent modifying the F plasmid so that the experiments wouldn’t require quite so much time. The diagram below shows a map of the E. coli F plasmid, similar to that shown in lecture. Sam is determined to “get rid of some of the excess DNA” present in the plasmid. Please keep in mind that the PI in the lab in which Sam works utilizes these F plasmids for interrupted mating experiments between Hfr and F- cells as well as for interrupted mating experiments involving F+…arrow_forwardUsually, bacteria only make tryptophan when tryptophan is absent or available in low concentration. However, a particular bacterial mutation makes tryptophan all the time whether or not tryptophan is present. What could explain this phenotype? A) the terminator hairpin is unable to form B) the antiterminator hairpin is unable to form C) trpE is mutated D) trpD is mutated E) trpA is mutatedarrow_forwardI read that vinyl chloride exposure is associated with an increased risk of a rare form of liver cancer (hepatic angiosarcoma), as well as brain and lung cancers, lymphoma, and leukemia. But my question is what gene(s) are being mutated by this type of taxic gas?arrow_forward
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