The way to use the given experimental apparatus to determine the average area occupied by a single lipid molecule in the monolayer. Introduction: Lipid is defined as a biomolecule which helps to store and provide energy. It is one of the main constituent of plasma membrane as it is made of lipid bilayer. It also serves as basic building for several hormones and bile acids.

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Chapter 11, Problem 1P

Summary Introduction

To analyze: The way to use the given experimental apparatus to determine the average area occupied by a single lipid molecule in the monolayer.

Introduction:

Lipid is defined as a biomolecule which helps to store and provide energy. It is one of the main constituent of plasma membrane as it is made of lipid bilayer. It also serves as basic building for several hormones and bile acids.

Expert Solution & Answer

Explanation of Solution

Explanation:

Single lipid molecule occupies the average area in the monolayer which could be calculated by dividing the surface area of the water at which the pressure raises sharply by the number of lipid molecules on the surface. The number of lipid molecules present on the surface can be calculated by multiplying the number of moles by the Avogadro’s number.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

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Chapter 11, Problem 1P

Summary Introduction

To analyze: The way to use the given experimental apparatus to determine the average area occupied by a single lipid molecule in the monolayer.

Introduction:

Lipid is defined as a biomolecule which helps to store and provide energy. It is one of the main constituent of plasma membrane as it is made of lipid bilayer. It also serves as basic building for several hormones and bile acids.

Expert Solution & Answer

Explanation of Solution

Explanation:

Single lipid molecule occupies the average area in the monolayer which could be calculated by dividing the surface area of the water at which the pressure raises sharply by the number of lipid molecules on the surface. The number of lipid molecules present on the surface can be calculated by multiplying the number of moles by the Avogadro’s number.

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Answer 3

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Chapter 11, Problem 1P

Summary Introduction

To analyze: The way to use the given experimental apparatus to determine the average area occupied by a single lipid molecule in the monolayer.

Introduction:

Lipid is defined as a biomolecule which helps to store and provide energy. It is one of the main constituent of plasma membrane as it is made of lipid bilayer. It also serves as basic building for several hormones and bile acids.

Expert Solution & Answer

Explanation of Solution

Explanation:

Single lipid molecule occupies the average area in the monolayer which could be calculated by dividing the surface area of the water at which the pressure raises sharply by the number of lipid molecules on the surface. The number of lipid molecules present on the surface can be calculated by multiplying the number of moles by the Avogadro’s number.

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Answer 4

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Chapter 11, Problem 1P

Summary Introduction

To analyze: The way to use the given experimental apparatus to determine the average area occupied by a single lipid molecule in the monolayer.

Introduction:

Lipid is defined as a biomolecule which helps to store and provide energy. It is one of the main constituent of plasma membrane as it is made of lipid bilayer. It also serves as basic building for several hormones and bile acids.

Expert Solution & Answer

Explanation of Solution

Explanation:

Single lipid molecule occupies the average area in the monolayer which could be calculated by dividing the surface area of the water at which the pressure raises sharply by the number of lipid molecules on the surface. The number of lipid molecules present on the surface can be calculated by multiplying the number of moles by the Avogadro’s number.

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Answer 6

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Answer 7

Chapter 11, Problem 1P

Summary Introduction

To analyze: The way to use the given experimental apparatus to determine the average area occupied by a single lipid molecule in the monolayer.

Introduction:

Lipid is defined as a biomolecule which helps to store and provide energy. It is one of the main constituent of plasma membrane as it is made of lipid bilayer. It also serves as basic building for several hormones and bile acids.

Expert Solution & Answer

Explanation of Solution

Explanation:

Single lipid molecule occupies the average area in the monolayer which could be calculated by dividing the surface area of the water at which the pressure raises sharply by the number of lipid molecules on the surface. The number of lipid molecules present on the surface can be calculated by multiplying the number of moles by the Avogadro’s number.

Answer 8

Chapter 11, Problem 1P

Summary Introduction

To analyze: The way to use the given experimental apparatus to determine the average area occupied by a single lipid molecule in the monolayer.

Introduction:

Lipid is defined as a biomolecule which helps to store and provide energy. It is one of the main constituent of plasma membrane as it is made of lipid bilayer. It also serves as basic building for several hormones and bile acids.

Expert Solution & Answer

Explanation of Solution

Explanation:

Single lipid molecule occupies the average area in the monolayer which could be calculated by dividing the surface area of the water at which the pressure raises sharply by the number of lipid molecules on the surface. The number of lipid molecules present on the surface can be calculated by multiplying the number of moles by the Avogadro’s number.

Answer 9

Chapter 11, Problem 1P

Summary Introduction

To analyze: The way to use the given experimental apparatus to determine the average area occupied by a single lipid molecule in the monolayer.

Introduction:

Lipid is defined as a biomolecule which helps to store and provide energy. It is one of the main constituent of plasma membrane as it is made of lipid bilayer. It also serves as basic building for several hormones and bile acids.

Answer 10

Expert Solution & Answer

Explanation of Solution

Explanation:

Single lipid molecule occupies the average area in the monolayer which could be calculated by dividing the surface area of the water at which the pressure raises sharply by the number of lipid molecules on the surface. The number of lipid molecules present on the surface can be calculated by multiplying the number of moles by the Avogadro’s number.