Biochemistry 410/411 Textbook - 5th Edition - Custom Texas A&M University
Biochemistry 410/411 Textbook - 5th Edition - Custom Texas A&M University
5th Edition
ISBN: 9781119302940
Author: Voet
Publisher: WILEY C
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Chapter 11, Problem 15E
Summary Introduction

To explain: The reason behind the lysozyme that cleaves the artificial substrate (NAG)4~4000 times more slowly than it cleaves (NAG)6.

Concept introduction: Lysozyme is an antimicrobial enzyme that forms a part of the innate immune system. Lysozyme attacks the protective cell walls of bacteria. It increases a healthy shelf life and ensures food safety.

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Imagine that aldolase can react with the seven carbon molecule Sedoheptulose-1,7-bisphosphate (below). Use the mechanism to predict the two products generated. Please draw out the stereochemistry in a fischer projection.
Sodium borohydride (NaBH4) is a potent inhibitor of aldolase. It is known to ONLY inhibit theenzyme when it is complexed with substrate. Treatment of the enzyme alone has no effect.What is the mechanism for this inhibition? Please draw out the mechanism and show how it inhibits this.
Show the fate of the proton on the 4-Oxygen molecule of F-1,6-BP. Please include a drawing showing the electron flow that occurs.

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Biochemistry 410/411 Textbook - 5th Edition - Custom Texas A&M University

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