The density ( ρ ) of avian tubercle bacillus DNA with the help of the given information is to be calculated. Concept introduction: The strands of the DNA molecule contain the complementary pairs of bases. The adenine is complementary nucleotide for thymine and cytosine is the complementary nucleotide for guanine. The adenine to guanine ratio must be equal to the thymine to cysteine ratio for double-stranded DNA. Isopycnic centrifugation is a technique that is used for the separation of molecules in solution on the basis of differences in their densities.

Question

Want to see the full answer?

Answer 1

Question

Chapter 11, Problem 12P

Interpretation Introduction

Interpretation:

The density(ρ)of avian tubercle bacillus DNA with the help of the given information is to be calculated.

Concept introduction:

The strands of the DNA molecule contain the complementary pairs of bases. The adenine is complementary nucleotide for thymine and cytosine is the complementary nucleotide for guanine. The adenine to guanine ratio must be equal to the thymine to cysteine ratio for double-stranded DNA.

Isopycnic centrifugation is a technique that is used for the separation of molecules in solution on the basis of differences in their densities.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

The preferred substrate is T because the enzyme half-saturates at 7.00 mM for T, but requires 28.0 mM for U, and 112 mM for S. b Question Content Area The rate constant k 2 with substrate S is 9.60×107 sec-1, with substrate T, k 2 = 6.00×104 sec-1, and with substrate U, k 2 = 2.40×106 sec-1. Calculate the catalytic efficiency with S, T, and U. Catalytic efficiency with S = Catalytic efficiency with T = Catalytic efficiency with U = Does enzyme A use substrate S, substrate T, or substrate U with greater catalytic efficiency?

Fumerase catalyzes the conversion of fumerate to malate. fumerate + H2O ⇋ malate The turnover number, kcat, for fumerase is 8.00×102 sec-1. The Km of this enzyme for fumerate is 5.00×10-3 μmol mL-1. a In an experiment using 2.00×10-3 μmol·mL-1, what is Vmax?

Suppose you wanted to make a buffer of exactly pH 7.00 using KH2PO4 and Na2HPO4. If the final solution was 0.18 M in KH2PO4, you would need 0.25 M Na2HPO4. Now assume you wish to make a buffer at the same pH, using the same substances, but want the total phosphate molarity ([HPO42−]+[H2PO−4]) to equal 0.20 M. What concentration of the Na2HPO4 would be required?