Anatomy & Physiology: The Unity of Form and Function
8th Edition
ISBN: 9781259277726
Author: Kenneth S. Saladin Dr.
Publisher: McGraw-Hill Education
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Chapter 10.1, Problem 4BYGO
Summary Introduction
Introduction:
The terms origin, belly and innervation are used to describe the anatomy and movements of the muscles.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 10 Solutions
Anatomy & Physiology: The Unity of Form and Function
Ch. 10.1 - Which muscles are included in the muscular system...Ch. 10.1 - Prob. 2AYLOCh. 10.1 - Prob. 3AYLOCh. 10.1 - Classification of muscles according to the...Ch. 10.1 - Prob. 5AYLOCh. 10.1 - Prob. 6AYLOCh. 10.1 - The flaw m origin-insertion terminology of muscle...Ch. 10.1 - The action of a muscle; how it relates to the...Ch. 10.1 - Prob. 9AYLOCh. 10.1 - Prob. 10AYLO
Ch. 10.1 - Prob. 11AYLOCh. 10.1 - Prob. 2BYGOCh. 10.1 - Prob. 3BYGOCh. 10.1 - Prob. 4BYGOCh. 10.1 - Prob. 5BYGOCh. 10.2 - Prob. 1AYLOCh. 10.2 - The orbicularis oculi, levator palpebrae...Ch. 10.2 - Prob. 3AYLOCh. 10.2 - Prob. 4AYLOCh. 10.2 - Prob. 5AYLOCh. 10.2 - Prob. 6AYLOCh. 10.2 - The intrinsic muscles of the tongue in general,...Ch. 10.2 - The temporalis, master, medial pterygoid, and...Ch. 10.2 - Prob. 9AYLOCh. 10.2 - Prob. 10AYLOCh. 10.2 - Prob. 11AYLOCh. 10.2 - Prob. 12AYLOCh. 10.3 - The diaphragm and the external intercostal,...Ch. 10.3 - Prob. 2AYLOCh. 10.3 - Prob. 3AYLOCh. 10.3 - The perineum, its two triangles, and their...Ch. 10.3 - Prob. 5AYLOCh. 10.3 - Prob. 6AYLOCh. 10.3 - Prob. 7AYLOCh. 10.3 - Prob. 13BYGOCh. 10.3 - Prob. 14BYGOCh. 10.3 - Prob. 15BYGOCh. 10.4 - Prob. 1AYLOCh. 10.4 - Muscles that act on the humerus, including the...Ch. 10.4 - The brachialis, biceps brachii, triceps brachii,...Ch. 10.4 - Prob. 4AYLOCh. 10.4 - The Palmaris longus, flexor carpi radialis, flexor...Ch. 10.4 - Prob. 6AYLOCh. 10.4 - The abductor pollicis longus, extensor pollicis...Ch. 10.4 - Prob. 8AYLOCh. 10.4 - Prob. 9AYLOCh. 10.4 - The midpalmar group of intrinsic hand muscles:...Ch. 10.4 - Name a muscle that inserts on the scapula and...Ch. 10.4 - Prob. 17BYGOCh. 10.4 - Prob. 18BYGOCh. 10.4 - Prob. 19BYGOCh. 10.4 - Prob. 20BYGOCh. 10.4 - Prob. 21BYGOCh. 10.5 - Prob. 1AYLOCh. 10.5 - Prob. 2AYLOCh. 10.5 - Prob. 3AYLOCh. 10.5 - Prob. 4AYLOCh. 10.5 - Muscles of the medial compartment of the thigh:...Ch. 10.5 - Prob. 6AYLOCh. 10.5 - The hamstring muscles of the posterior compartment...Ch. 10.5 - Prob. 8AYLOCh. 10.5 - Prob. 9AYLOCh. 10.5 - Prob. 10AYLOCh. 10.5 - Prob. 11AYLOCh. 10.5 - Prob. 12AYLOCh. 10.5 - Prob. 13AYLOCh. 10.5 - Prob. 14AYLOCh. 10.5 - List the major actions of the muscles of the...Ch. 10 - Prob. 1TYRCh. 10 - Prob. 2TYRCh. 10 - Prob. 3TYRCh. 10 - Prob. 4TYRCh. 10 - Which of these muscles of the pelvic floor is the...Ch. 10 - Prob. 6TYRCh. 10 - Both the hands and feet ate acted upon by a muscle...Ch. 10 - Prob. 8TYRCh. 10 - Prob. 9TYRCh. 10 - Which of the following muscles raises the upper...Ch. 10 - Prob. 11TYRCh. 10 - Prob. 12TYRCh. 10 - Prob. 13TYRCh. 10 - Prob. 14TYRCh. 10 - Connective tissue bands called ________ prevent...Ch. 10 - Prob. 16TYRCh. 10 - Prob. 17TYRCh. 10 - A muscle that works with another to produce the...Ch. 10 - Prob. 19TYRCh. 10 - Prob. 20TYRCh. 10 - Prob. 1BYMVCh. 10 - Prob. 2BYMVCh. 10 - Prob. 3BYMVCh. 10 - Prob. 4BYMVCh. 10 - Prob. 5BYMVCh. 10 - Prob. 6BYMVCh. 10 - Prob. 7BYMVCh. 10 - Prob. 8BYMVCh. 10 - Prob. 9BYMVCh. 10 - Prob. 10BYMVCh. 10 - Prob. 1WWTSCh. 10 - Prob. 2WWTSCh. 10 - Prob. 3WWTSCh. 10 - Prob. 4WWTSCh. 10 - Prob. 5WWTSCh. 10 - Prob. 6WWTSCh. 10 - Prob. 7WWTSCh. 10 - Prob. 8WWTSCh. 10 - Prob. 9WWTSCh. 10 - The tibialis anterior and tibialis posterior are...Ch. 10 - Prob. 1TYCCh. 10 - Removal of cancerous lymph node from the neck...Ch. 10 - Prob. 3TYCCh. 10 - Prob. 4TYCCh. 10 - Prob. 5TYCCh. 10 - Prob. 1BYGOCh. 10 - Prob. 2BYGOCh. 10 - Prob. 3BYGOCh. 10 - Prob. 4BYGOCh. 10 - Prob. 5BYGOCh. 10 - Prob. 6BYGOCh. 10 - Prob. 7BYGOCh. 10 - Prob. 8BYGOCh. 10 - BEFORE YOU GO ON
9. Distinguish between the...Ch. 10 - Prob. 10BYGOCh. 10 - Prob. 11BYGOCh. 10 - Prob. 12BYGOCh. 10 - 22. In the middle of stride, you have one foot on...Ch. 10 - Prob. 23BYGOCh. 10 - Prob. 24BYGOCh. 10 - Prob. 25BYGO
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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