Chapter 10, Problem 10TYU

SCIENTIFIC INQUIRY • MAKE CONNECTIONS The following diagram represents an experiment with isolated thylakoids. The thylakoids were first made acidic by soakingthem in a solution at pH 4. After the thylakoid space reached pH 4, the thylakoids were transferred to a basic solution at pH 8. The thylakoids then made ATP in the dark. (See Concept 3.3 to review pH.)

Draw an enlargement of part of the thylakoid membrane in the beaker with the Solution at pH 8. Draw ATP synthase. Label the areas of high H⁺ concentration and low H⁺ concentration. Show the direction protons flow through the enzyme, and show the reaction where ATP is synthesized. Would ATP end up in the thylakoid or outside of it? Explain why the thylakoids in the experiment were able to make ATP in the dark.