Your research lab is interested in determining the phenotype of three of your patients (DNA 1, DNA 2, and DNA 3) for the gene TNF alpha, known to exist in two isoforms (wild-type TNF1 and mutant TNF2). Using gel electrophoresis to isolate the patients DNA, you obtain the results of the experiment as depicted in the left image below. Interested in your results, your boss asks you to perform the experiment again, to ensure your results are consistent. Upon performing the experiment again, you obtain the results as depicted in the right image below.. Assuming the left image is what you should have obtained, which of the following conclusions is correct and indicates potential errors that could have occurred in the right image. You accidentally added the TNF1 and TNF2 control DNA samples to the TNF1 lane, the TNF2 lane incorrectly added TNF2 control only, you forgot to add DNA 2 sample to the DNA 1 lane, you accidentally added DNA 1 sample to the DNA 2 lane, and you accidentally added DNA 2 sample to the DNA 3 lane. O You accidentally added the TNF1 and TNF2 control DNA samples to the TNF1 lane, the TNF2 lane correctly added TNF2 control only, you forgot to add DNA 1 sample to the DNA 1 lane, you accidentally added DNA 3 sample to the DNA 2 lane, and you accidentally added DNA 2 sample to the DNA 3 lane. You accidentally added the TNF1 and TNF2 control DNA samples to the TNF1 lane, the TNF2 lane correctly added TNF2 control only, you forgot to add DNA 3 sample to the DNA 1 lane, you accidentally added DNA 1 sample to the DNA 2 lane, and you accidentally added DNA 2 sample to the DNA 3 lane. You accidentally added the TNF1 and TNF2 control DNA samples to the TNF1 lane, the TNF2 lane correctly added TNF2 control only, you forgot to add DNA 2 sample to the DNA 1 lane, you accidentally added DNA 1 sample to the DNA 2 lane, and you accidentally added DNA 2 sample to the DNA 3 lane. 177 T
Your research lab is interested in determining the phenotype of three of your patients (DNA 1, DNA 2, and DNA 3) for the gene TNF alpha, known to exist in two isoforms (wild-type TNF1 and mutant TNF2). Using gel electrophoresis to isolate the patients DNA, you obtain the results of the experiment as depicted in the left image below. Interested in your results, your boss asks you to perform the experiment again, to ensure your results are consistent. Upon performing the experiment again, you obtain the results as depicted in the right image below.. Assuming the left image is what you should have obtained, which of the following conclusions is correct and indicates potential errors that could have occurred in the right image. You accidentally added the TNF1 and TNF2 control DNA samples to the TNF1 lane, the TNF2 lane incorrectly added TNF2 control only, you forgot to add DNA 2 sample to the DNA 1 lane, you accidentally added DNA 1 sample to the DNA 2 lane, and you accidentally added DNA 2 sample to the DNA 3 lane. O You accidentally added the TNF1 and TNF2 control DNA samples to the TNF1 lane, the TNF2 lane correctly added TNF2 control only, you forgot to add DNA 1 sample to the DNA 1 lane, you accidentally added DNA 3 sample to the DNA 2 lane, and you accidentally added DNA 2 sample to the DNA 3 lane. You accidentally added the TNF1 and TNF2 control DNA samples to the TNF1 lane, the TNF2 lane correctly added TNF2 control only, you forgot to add DNA 3 sample to the DNA 1 lane, you accidentally added DNA 1 sample to the DNA 2 lane, and you accidentally added DNA 2 sample to the DNA 3 lane. You accidentally added the TNF1 and TNF2 control DNA samples to the TNF1 lane, the TNF2 lane correctly added TNF2 control only, you forgot to add DNA 2 sample to the DNA 1 lane, you accidentally added DNA 1 sample to the DNA 2 lane, and you accidentally added DNA 2 sample to the DNA 3 lane. 177 T
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Your research lab is interested in determining the phenotype of three of your patients (DNA 1, DNA
2, and DNA 3) for the gene TNF alpha, known to exist in two isoforms (wild-type TNF1 and mutant
TNF2). Using gel electrophoresis to isolate the patients DNA, you obtain the results of the
experiment as depicted in the left image below. Interested in your results, your boss asks you to
perform the experiment again, to ensure your results are consistent. Upon performing the
experiment again, you obtain the results as depicted in the right image below.. Assuming the left
image is what you should have obtained, which of the following conclusions is correct and indicates
potential errors that could have occurred in the right image.
You accidentally added the TNF1 and TNF2 control DNA samples to the TNF1 lane, the TNF2 lane incorrectly
added TNF2 control only, you forgot to add DNA 2 sample to the DNA 1 lane, you accidentally added DNA 1
sample to the DNA 2 lane, and you accidentally added DNA 2 sample to the DNA 3 lane.
You accidentally added the TNF1 and TNF2 control DNA samples to the TNF1 lane, the TNF2 lane correctly
added TNF2 control only, you forgot to add DNA 1 sample to the DNA 1 lane, you accidentally added DNA 3
sample to the DNA 2 lane, and you accidentally added DNA 2 sample to the DNA 3 lane.
You accidentally added the TNF1 and TNF2 control DNA samples to the TNF1 lane, the TNF2 lane correctly
added TNF2 control only, you forgot to add DNA 3 sample to the DNA 1 lane, you accidentally added DNA 1
sample to the DNA 2 lane, and you accidentally added DNA 2 sample to the DNA 3 lane.
You accidentally added the TNF1 and TNF2 control DNA samples to the TNF1 lane, the TNF2 lane correctly
added TNF2 control only, you forgot to add DNA 2 sample to the DNA 1 lane, you accidentally added DNA 1
sample to the DNA 2 lane, and you accidentally added DNA 2 sample to the DNA 3 lane.
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