You treat cells with 2,4 dinitrophenol (DNP). This compound creates a temporary channel in the inner membrane of the mitochondria though the protons can move. Which of the following would you observe in these cells? Select all that apply The cells do not synthesize ATP in the presence of DNP The cells synthesize ATP in the presence of DNP The cells reduce oxygen in the presence of DNP The cells generate carbon dioxide in the presence of DNP

You treat cells with 2,4 dinitrophenol (DNP). This compound creates a temporary channel in the inner membrane of the mitochondria though the protons can move. Which of the following would you observe in these cells? Select all that apply The cells do not synthesize ATP in the presence of DNP The cells synthesize ATP in the presence of DNP The cells reduce oxygen in the presence of DNP The cells generate carbon dioxide in the presence of DNP

Human Physiology: From Cells to Systems (MindTap Course List)

9th Edition

ISBN:9781285866932

Author:Lauralee Sherwood

Publisher:Lauralee Sherwood

Chapter2: Cell Physiology

Section: Chapter Questions

Problem 2TAHL

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You are studying the process of oxidative phosphorylation in the lab. You isolate several mitochondria from cells and place them in a buffered solution with a low pH and observe that the mitochondria begin to synthesize ATP. Which of the following is the best explanation for this observation? F It increases the conc. of OH-, causing the mitochondria to pump H+ to the intermembrane space. It increases the diffusion of H+ from the mitochondrial matrix to the intermembrane space. It increases the diffusion of H+ from the intermembrane space to the matrix. It increases the conc. of OH- in the mitochondrial matrix.
An animal cell, roughly cubical in shape with side length of 10 μm, uses 109 ATP molecules every minute. assume that the cell replaces this ATP by the oxidation of glucose according to the overall reaction 6O2 + C6H12O6 →6CO2 + 6H2O and that complete oxidation of each glucose molecule produces 30 ATP molecules. how much oxygen does the cell consume every minute? How long will it take before the cell has used up an amount of oxygen gas equal to its own volume?
The standard free energy change for ATP hydrolysis is -7.3kcal/mol. The free energy change under typical cellular conditions is about -12kcal/mol. Why is there a difference? O The hydrolysis of ATP must be more difficult in the cellular environment O There is more ATP and less ADP in the cell O The cellular environment is anaerobic so O2 levels are reduced O The enzymes involved in the reaction are trapped in organelles
Which of the following statements about oxidative phosphorylation is correct? O H+ ions are transferred from Complex I or Complex II to ATP synthase where ATP production occ O Proton pumps transfer electrons from the cytosol to the mitochondrial matrix as electrons are tra O The chemical and electrical gradient is established between the intermembrane space and the ma electron carriers. O ATP synthase pumps electrons back to the intermembrane space as a consequence of electrocher mitochondrial matrix. • Previous
How much energy is required to pump a mole of protons in a mitochondria with the following conditions? pH of matrix = 8.2, pH of intermembrane space 7.4, membrane potential of - 65 mV (N-side relative to P-side), temperature = 37C. Express answer to the nearest 0.1 kJ/mole.
Cyanide poisoning inhibits aerobic respiration at cytochrome c oxidase. Which of the following is NOT a result of cyanide poisoning at the cellular level? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a b с d e Oxygen is reduced to water The rate of glycolysis increases Cells are forced to switch to anaerobic respiration The electron transport chain is not completed None of the above Answered K Open in Reading View ✔Posubmit
ATP powers the following cellular work, except o a. Active transport across the membrane O b. Diffusion down the gradient O c. None of the choices o d. Movement of motor proteins e. Anabolic reactions
In the presence of excess oxygen, a complete oxidation of seven molecules of glucose into carbon dioxide and water, by a yeast cell, would produce approximately ATP molecules via oxidative phosphorylation only. (Consider NADH = 3 ATP and FADH2 = 2 ATP)
A 75 kg person doing light work requires about 3000 kcal of food energy perday, 40% of which is actually used by the body’s cells. (The other 60% is lostas heat and in waste products.) Before being used by the cells, effectively allof this energy is stored in ATP, which is then cleaved into ADP and PO3−2 , with the release of 12.5 kcal per mole of ATP.(a) How many moles of ATP are turned over per day in this fashion? Whatmass of ATP does this correspond to? (The molecular weight of ATP is507 g/mol.)(b) The body actually contains approximately 5 g ATP. Estimate the averagerecycle time for an ATP molecule. You see that it is much more efficientto reuse ADP rather than to synthesize it de novo (from scratch).
without oxygen US V 11 glucose G 2 13 14 pyruvate with oxygen acetyl Col CO₂ CO₂ Using the diagram above, fill in the chart below for a summary of cellular respiration taking place in the mitochondria. X₂ X² но B I 2 4 electron transport system C > X Q 흐트 E Summary of Cellular Respiration Name of Location Brief Is ATP process of process Description produced? I E D
The concentration of glucose in your circulatory system is maintained near 5.0 mM by the actions of the pancreatic hormones glucagon and insulin. Glucose is imported into cells by protein transporters that are highly specific for binding glucose. Inside the liver cells the imported glucose is rapidly phosphorylated to give glucose-6-phosphate (G-6-P). This is an ATP-dependent process that consumes 1 mol ATP per mol of glucose. Given the steady-state intracellular concentrations below, calculate the theoretical maximum concentration of G-6-P inside a liver cell at 37 °C, pH = 7.2 when the glucose concentration outside the cell (i.e., [glucoseloutside) is 5.0 mM: ATP = 4.7 mM; ADP = 0.15 mM; P, = 6.1 mM For: ATP + H,O ADP + P + H* AG" = -30.5 kJ/mol and G-6-P + H,0 -→ Glucose + P AG" = -13.8 kJ/mol The glucose phosphorylation reaction is ATP + glucosenside » ADP + glucose-6-phosphate + H+
The production of a hydrogen ion concentration gradient powers the production of ATP in both photosynthesis and cellular respiration. 2,4-Dinitrophenol (DNP) is an organic compound that 'undoes' the hydrogen ion concentration gradient without the production of ATP. It does this by increasing the membrane permeability to hydrogen ions. One use of DNP is as a herbicide.For a brief period in the 1930s, DNP was marketed as a diet pill. Because DNP prevents the production of ATP, the human body will begin to use alternate forms of energy. The result is an increase in the metabolism of fats in the body, thus reducing total body fat. In the presence of DNP, the energy that normally would be converted to ATP for use in the cells is converted to heat instead, causing dangerously high body temperatures.DNP is classified as an illegal substance in Canada and the U.S. although it is still marketed as a commercial chemical. In recent years, several deaths have been reported, primarily in the…