You prepare a squid giant axon and inject it with a tiny volume of solution containing 0.1 M NaCl and 0.1 M KCl in which both the Na+ and K+ ions are radioactively labeled. The two labeled ions are now inside the axon. You place this axon in a beaker containing seawater medium. The voltage gated sodium and voltage gated potassium channels are completely blocked using specific inhibitors - so they are closed If you now remove the voltage gated potassium channel inhibitor and keep the voltage gated sodium channel blocked, which of the radioactively labeled ions would you expect to appear within the seawater medium, while the neuron remained at rest (no stimulation of axon). Please explain If you now remove both the sodium and potassium voltage gated channel inhibitors - which of the radioactively labeled ions would you expect to appear most rapidly within the seawater medium, after the neuron had been stimulated to conduct a number of action potentials? Please explain
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
You prepare a squid giant axon and inject it with a tiny volume of solution containing 0.1 M NaCl and 0.1 M KCl in which both the Na+ and K+ ions are radioactively labeled. The two labeled ions are now inside the axon. You place this axon in a beaker containing seawater medium. The voltage gated sodium and voltage gated potassium channels are completely blocked using specific inhibitors - so they are closed
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If you now remove the voltage gated potassium channel inhibitor and keep the voltage gated sodium channel blocked, which of the radioactively labeled ions would you expect to appear within the seawater medium, while the neuron remained at rest (no stimulation of axon). Please explain
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If you now remove both the sodium and potassium voltage gated channel inhibitors - which of the radioactively labeled ions would you expect to appear most rapidly within the seawater medium, after the neuron had been stimulated to conduct a number of action potentials? Please explain
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