You inserted your gene of interest in pBR322 as illustrated below. You spliced the vector and insert using BamHI. After ligation and transformation, describe your screening procedures. Scal 3844 Pvul 3733 Pstl 3607 Vspl 3537 Eco311 3433 Eam11051 3361 Cail 2884. rep (pMB1) bla (Ap) 3293 Bsul5l 23 EcoRI, Xapl 4359, Hindi 29 Eco321 185 Aatil 4284, Sspl 4168 3133 Afill, Pscl 2473 2519 Lgul 2350/ 4153 pBR322 4361 bp Ndel 2295/ Bst11071 2244/ 2106 1915 Bspol, Nhel 229 rop Pull 2064 Pfol 2117 Esp31 2122 Ppu211 2225 Psyl 2217 BamHI 375 Tstl 389 SgrAl 409 tet (Tc) 1276 Pael 562 Xagl 622 Sall 651 Boxl 712 Eco521 939 Bsp681 972 Bvel 1063 Mva12691 1353 Eco1301 1309 Eco881 1425 Misl 1444 Bpu101 1580 Bsgl 1650 Kpn21 1664 BseJl 1668

Q: You have isolated a cDNA clone encoding a protein of interest in a higher eukaryote. This cDNA clone…

A: cDNA  It is known as complementary DNA and made up of reverse transcription of mRNA.

Q: Two homologous chromosomes in ES cells are depicted below with the portion of your gene of interest…

A: CRISPR/Cas9 creates specific double-stranded breaks at the target locus that trigger DNA repair…

Q: In a single-locus homology-directed repair (HDR), CRISPR/Cas9 machinery makes breaks in the dsDNA at…

A: 2

Q: Describe the banding pattern seen in the gel. What does that tell you about the role of annealing…

A: Gel electrophoresis is a laboratory technique for separating charged molecules such as DNA, RNA, and…

Q: Using figure (a) above as guide, list the five components in the CRISPR-Cas9 bacterial defense…

A: CRISPR is a acronym for Clustered regularly interspaced short palindromic repeats. It forms a very…

Q: How can I develop a vector to express a bacterial gene (2,500 bp in size) in E. coli. How do I know…

A: Hi! As you have posted multiple questions, I will be answering the first two questions for you. If…

Q: What evidence demonstrates that CRISPR-Cas is an adaptive immune system defense against viruses?

A: A virus is a very small submicroscopic agent that is known for causing infections in its host…

Q: For a coding gene, purifying (negative) selection will result in more synonymous (silent) site…

A: Purifying selection is the most predominant form of natural selection or negative selection. As it…

Q: When a genome is edited using the CRISPR/Cas9 system, a knockout allele can be produced if DNA is…

A: CRISPR: CRISPR  is a newly emerging tool that is used by scientists to alter the genome structure by…

Q: Consider the following types of cells: F+, F-, Hfr, and F’ cells. Which of these four types of cells…

A:

Q: name and briefly describe two methods you could use to identify the changes in DNA sequence found in…

A: DNA mutations can have a wide range of consequences, including the development of genetic disorders,…

Q: Prior to the publication of the article from which this assay comes from (picture), the Cas9…

A: SDS-PAGE Analysis of Cas9 Orthologsa. Experimental Question and HypothesisResearch Question: The…

Q: How was it experimentally proven that the spacer sequences in the CRISPR locus were the elements…

A: CRISPR-cas system provides an adaptive defence mechanism to the prokaryotes which acquires…

Q: MDF57 is a 110kDa DNA binding protein which has some functional roles in the development of…

A: Mutation is any change in the genetic setup of the cell such as change in the sequence, structure or…

Q: A cloned Bam HI DNA fragment, 3.0 kb long, was isolated in preparation for sequence determination.…

A: Restriction mapping is done by analysing and arranging the fragments that are generated by the use…

Q: Can you suggest how we could have determined whether Bill’s aunts were carriers of the mutant BTK…

A: The Bruton tyrosine kinase is important for the growth and maturation of B cells by some change and…

Q: What is the difference between using genome build hg18 and hg19 in Genome-wide SNP arrays?

A: SNP (Single nucleotide protein) array is a type of DNA (deoxyribonucleic acid) microarray which is…

Q: What is it about the Cas9 protein that makes it an efficient DNAediting tool?

A: DNA and RNA are the genetic elements present in almost all cells. DNA encodes for proteins through…

Q: Can you develop a siRNA-based technology to treat COVID-19? If so, please provide the details on the…

A: YES,according to me this insight seams to be reasonable. siRNA or small interfering RNA is a non…

Q: Your mutagenized sample showed 523 CFU after plating 100uL of media on the 10^3 dilution plate.  The…

A: CFU/ml = CFU/(Dilution × Volume plated)  CFU stands for colony forming units.

Q: Which restriction enzyme is best suited for cloning in pUC8?

A: The restriction enzymes are specific endonuclease that cut the double stranded DNA from the middle…

Q: 1) Enhanced GFP protein has: An excitation peak at 488nm and emits maximally at 507nm. An excitation…

A: Answer 1.  Answer. 1). Enhanced GFP protein has an excitation peak at 488nm and emits maximally…