You inoculate a species of bacteria on a MacConkey agar which normally shows positive for growth and ability to breakdown lactose but does not. After talking to your lab technician about this you learn the MacConkey agar was not made the correct way. Please discuss what method(s) can be performed and the results that would help confirm what the MacConkey agar would normally show.

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Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.