a) explain in your own words how to perform specialized media tests to find an unknown bacteria

Q: A glass flask is rinsed and autoclaved after use. It was then filled with sterile media in a sterile…

A: Introduction Microbes or microorganisms may be found on almost every surface, in the air we breathe,…

Q: layer

A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…

Q: The number of bacteria in a sample can also be quantified with other techniques, such as examination…

A: In microbiology laboratories the number of microorganisms present in a particular sample is…

Q: Which of the following describes the usefulness of a Gram stain.  Select all that apply. a. A Gram…

A: A popular microbiological method called "Gram staining" is used to separate and categorize bacteria…

Q: Based on the following image, which concentration of antibiotic would be considered the minimum…

A: Minimum bactericidal concentration (MBC) is defined as the lowest concentration in which an…

Q: a food sample with an unknown number of contaminating bacteria was serial diluted to 10-6. the 10-6…

A: Serial dilution is a process of diluting a particular sample in a solution. The purpose of doing a…

Q: Why do cells need to be kept in the freezing devices first then transferred to liquid nitrogen…

A: Rapid freezing of cells can cause ice crystals large crystals can be formed in which cells are…

Q: Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture…

A: In microbiology, culture refers to microbial growth of a certain species maintained in controlled…

Q: which of these tests (i.e gram stain, acid fast stain, endospore, oxidation to fermentation,…

A: A dichotomous key is a tool designed by scientists to determine the identity of the organism. Here,…

Q: As part of an experiment where absorbance values are measured using a spectrophotometer, you are…

A: The bacteria growth uses mitotic cell divisions. The bacterial population grows until the nutrients…

Q: A colleague performs a smear prep from a colony on an agar plate. Without staining it, he looks at…

A: Gram staining is a common laboratory technique in microbiology that is used to differentiate…

Q: Lactose fermentation

A: The dichotomous key is the tool for the identification of unknown organism among the group. Choice…

Q: Complete the table below with the observations: include amount of growth (-, +, ++, +, ++), colonial…

A: Organism / MediaNutrient Agar (NA)Eosin Methylene Blue (EMB)Mannitol Salt Agar (MSA)Escherichia…

Q: he image below is the results of the Nutrient agar plate inoculated with the kitchen ounter sample…

A: Colony morphology is used by microbiologists to identify and characterized the different bacterial…

Q: An attempt to transfer bacteria into new media during the death (Decline) phase of a culture…

A: Microbial cultures, also known as microbiological cultures, are made by allowing microbial organisms…

Q: In Microbiology What does a metallic green sheen indicate on an EMB (Eosin Methylene Blue…

A: Microbial isolation is a technique in which microbes of interest are seperated out for the purpose…

Q: You inoculate a species of bacteria on a MacConkey agar which normally shows positive growth and the…

A: MacConkey Agar (MAC) is selective and differential agar that is used to isolate non-fastidious…

Q: Collecting data that can then be transformed into a bacterial growth curve is usually done using a…

A: A spectrophotometer is an instrument used to measure the amount of light absorbed by a sample. The…

Q: In the laboratory, autoclaving is required to prepare media for the growth of What type of method of…

A: The most effective method by which we sterilize the laboratory instruments is known as autoclaving.

Q: You begin with an overnight culture of approximately 108 organisms/ml and need a concentration of…

A: In an Overnight culture of the bacteria, their growth factor depends upon the type of bacteria we…

Q: identify the organisms in  an unknown culture. You find that the culture contains two gramnegative…

A: A culture is a medium to grow bacteria and other microorganisms  It provides the bacteria with…

Q: A microbiology student was given a mixed culture of two different gram positive bacteria species to…

A: In Microbiology, we grow bacteria on culture medium. Culture media contains specific mixture of…

Q: Using a dilution series is a way to transfer the very small volumes of original culture necessary to…

A: Answer -To determine the volume of the original bacterial culture required to obtain a specific…

Q: Imagine you made a single kirby bauer plate to determine if a given antibiotic were effective for…

A: Introduction Antibiotics, also called antibacterials, are drugs that kill or slow the growth of…

Q: Describe how each of the following would appear when grown on EMB agar: a. Escherichia coli b.…

A: EMB stands for Eosin Methylene Blue. This media is used as a seletive stain for the gram negative…

Q: For a laboratory exercise of basic techniques in microbiology: Gram stain and Microscopy. What are…

A: "Gram staining is important in microbiology because it allows scientists to quickly and easily…

Q: Name of Organism: Citrobacter freundii Туре of Medium Color Before Incubation Color After (Same for…

A: Citrobacter freundii is a gram-negative bacteria that is rod-shaped and belongs to the bacterial…

Q: which of these tests (i.e gram stain, acid fast stain, endospore, oxidation to fermentation,…

A: The question asks about the test that can be used on the disease-causing organism below that can…

Q: A researcher is planning to plant antibiotic-resistant Escherichia coli pure bacteria sample in…

A: Culturing the bacteria on a solid media involves several steps in which the major step is…

Q: Identify the oxidase-negative, gram negative bacillus isolated from the blood cultures of a…

A: Gram-negative bacteria are those that do not retain the crystal violet stain after gram staining.…

Q: Why is it best to use sterile distilled water in the preparation of microbial suspension and dry…

A: Smear preparation is the first step carried out during the staining procedure.

Q: Provide comparison and illustration of expected growth curves from a pure culture with the…

A: 1. The growth curve from a pure culture represents the number of live cells in a bacterial…

Q: You obtain a 1 mL sample of contaminated drinking water in order to calculate the number of  fecal…

A: The evaluation of fecal microscopic organisms in water could be a critical public well-being measure…

Q: A microbiologist has isolated a new strain of bacteria, and is trying to characterize aspects of its…

A: Psychrophilic bacteria are cold loving bacteria that grows and reproduce at lower temperatures…

Q: For each of the following situations, describe how bacteria on these growth media would appear on…

A: In this examination, we will explore the appearance of bacterial growth on various growth media…

Q: explain why each component is used in a typical culture media ?

A: The type of microorganism utilised in the fermentation process affects the culture media needs,…

Q: How does smear preparation of cells from a liquid medium differ from preparation of cells from a…

A: Water is used to turn solid material containing cells into a liquid condition, which is then used to…

Q: Joseph, being a know-it-all, told Mary that her method would not include all microbes. To…

A: Joseph and Mary had a disagreement about the effectiveness of Mary's method for counting microbes in…

Q: You are hired in one of the microbiology labs in Memphis and your first assignment to identify…

A: 9. Gram-negative bacteria are characterized by their cell walls, which have a thinner layer of…

Q: not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical…

A: Foot rash can occur as a result of an allergic reaction or infection. For example in athletes food…

Q: Note that it is not appropriate to self-diagnose outside of a medical context and this is a…

A: Introduction Bacteria are prokaryotic unicellular organisms. They are under the kingdom Monera.…