You have two E. coli strains, XL10 and K12. XL10 is ampicillin resistant, or AmpR, and can grow on lab media plates in the presence ampicillin. K12 is ampicillin sensitive, or AmpS, and cannot grow on lab media plates in the presence of ampicillin. You suspect that the AmpR gene is carried on a plasmid in XL10, rather than on its chromosome, and want to design an experiment to test this hypothesis. In designing your experiment, you may assume that you have access to the following materials: all reagents, plates, plasmids, and equipment used in the lab this semester Overnight cultures of XL10 & K12 Competent cells of XL10 & K12 a. Describe briefly the steps of a simple experiment that you could perform to test the hypothesis that the AmpR gene is carried on a plasmid in XL10 and not on its chromosome. (NOTE: no need for detailed procedure, simply state what you would do is sufficient). b. What specific experimental result will tell you that your hypothesis is correct?

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Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.