You have the following sequence reads from a genomic clone of the Homo sapiens genome: Read 1: ATGCGATCTGTGAGCCGAGTCTTTA Read 2: AACAAAAATGTTGTTATTTTTATTTCAGATG Read 3: TTCAGATGCGATCTGTGAGCCGAG Read 4: TGTCTGCCATTCTTAAAAACAAAAATGT Read 5: TGTTATTTTTATTTCAGATGCGA Read 6: AACAAAAATGTTGTTATT a. Use these six sequence reads to create a sequence contig of this part of the H. sapiens genome. b. Translate the sequence contig in all possible reading frames. c. Go to the BLAST page of the National Center for Biotechnology Information, or NCBI (http://www.ncbi .nlm.nih.gov/BLAST/, Appendix B) and see if you can identify the gene of which this sequence is a part by using each of the reading frames as a query for protein– protein comparison (BLASTp).
You have the following sequence reads from a genomic
clone of the Homo sapiens genome:
Read 1: ATGCGATCTGTGAGCCGAGTCTTTA
Read 2: AACAAAAATGTTGTTATTTTTATTTCAGATG
Read 3: TTCAGATGCGATCTGTGAGCCGAG
Read 4: TGTCTGCCATTCTTAAAAACAAAAATGT
Read 5: TGTTATTTTTATTTCAGATGCGA
Read 6: AACAAAAATGTTGTTATT
a. Use these six sequence reads to create a sequence
contig of this part of the H. sapiens genome.
b. Translate the sequence contig in all possible reading
frames.
c. Go to the BLAST page of the National Center for
Biotechnology Information, or NCBI (http://www.ncbi
.nlm.nih.gov/BLAST/, Appendix B) and see if you can
identify the gene of which this sequence is a part by
using each of the reading frames as a query for protein–
protein comparison (BLASTp).
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