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You have a 15 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 8.24. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. At the end of the incubation period, you measure the pH again and find that it has decreased to 6.69. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in the original 15 mL sample. CH3 -CH₂-CH₂-N-CH₂ CH3 CH₂-C -0-CH₂- Acetylcholine acetycholine in original sample: TOOLS x10x H₂O CH3 HO–CH,—CH,—*N–CH, * CH,—C−0 + H+ CH₂--0 Choline CH3 Acetate nmol

You have a 15 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 8.24. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. At the end of the incubation period, you measure the pH again and find that it has decreased to 6.69. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in the original 15 mL sample. CH3 -CH₂-CH₂-N-CH₂ CH3 CH₂-C -0-CH₂- Acetylcholine acetycholine in original sample: TOOLS x10x H₂O CH3 HO–CH,—CH,—*N–CH, * CH,—C−0 + H+ CH₂--0 Choline CH3 Acetate nmol

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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You have a 15 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 8.24. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. At the end of the incubation period, you measure the pH again and find that it has decreased to 6.69. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in the original 15 mL sample. CH₂ H₂O CH₂-8-0-CH₂-CH₂-4H-CH HO–CH, CH, NCH, A CH, CH,—C−O−CH,—CH,−*N–CH, → HO–CH,—CH,—*N–CH, * CH,—C CH3 Acetylcholine acetycholine in original sample: x10 CH3 TOOLS Choline Acetate + H+ nmol
Transcribed Image Text:You have a 15 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 8.24. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. At the end of the incubation period, you measure the pH again and find that it has decreased to 6.69. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in the original 15 mL sample. CH₂ H₂O CH₂-8-0-CH₂-CH₂-4H-CH HO–CH, CH, NCH, A CH, CH,—C−O−CH,—CH,−*N–CH, → HO–CH,—CH,—*N–CH, * CH,—C CH3 Acetylcholine acetycholine in original sample: x10 CH3 TOOLS Choline Acetate + H+ nmol
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