You digested genomic dna with two restriction enzymes, Hinf I and Rsa I. These enzymes are used because: a. These enzymes recognize sequences TTAGGG present in TRF (terminal restriction fragment) at the ends of human chromosomes. b. These enzymes do not recognize sequences present in human telomeres' TRF (terminal restriction fragment). c. These enzymes are not expensive and very stable, making it possible to use them in a student laboratory course. d. These enzymes will not cut human genomic DNA

Q: Short tandem repeats (STR) profiling is based on O A. the fact that many foods are being genetically…

A: The method of DNA profiling used today is based on PCR and uses simple sequences or short tandem…

Q: What is the principle of the Restriction Fragment Length Polymorphism (RFLP) in the diagnosis of…

A: Restriction fragment length polymorphism (RFLP) : it is a method used in genome mapping and…

Q: A researcher is interested in using the in vitro technique of bisulfite conversion to confirm the…

A: DNA methylation is a chemical change that frequently takes place in the setting of CpG dinucleotides…

Q: When molecular biologists carry out a PCR, they have a range of different DNA polymerase enzymes on…

A: Polymerase chain reaction (PCR) is a frequently used approach for rapidly producing millions or even…

Q: Match Column A with Column B F. v Analysis of genome-wide patterns of gene expression F. v…

A: Recombinant DNA technology - It is a technology which forms the desired DNA sequence of interest by…

Q: Explain how the SNP changes the recognition sequence for the restriction enzyme and affects its…

A: *Single nucleotide polymorphisms (SNPs) involve variation of a single base pair and or SNPsbin the…

Q: You are trying to clone a gene, You have successfully isolated it from the genomic DNA of an…

A: The term DNA cloning is associated with a process that plays an important role in making copies of a…

Q: You have a DNA sequence of 1800 bp. To characterize it, you decide to make a restriction map.…

A: Restriction mapping is a process that helps to determine the location of the restriction site. The…

Q: What is the expected frequency of the restriction site 5′−GGCC−3′ in a genome? A. Once every 4…

A:

Q: Describe the nature of recognition sites for restriction enzymes and the nature of the ends of the…

A: Vast uses of endonuclease activity of restriction enzyme found in the biotechnology for gene…

Q: a. Using nucleotide letters, show the kind of cut that could be made on a DNA molecule to…

A: The plasmid is a small, circular deoxyribonucleic acid apart from the main bacterial DNA. Plasmids…

Q: The question is:

A: Approach to solving the question: I hope this helps!! Detailed explanation: Examples: Key…

Q: (RE) recognition sites marked with arrows. A spontaneous mutation (insertion) occurred in DNA sample…

A: Answer ::

Q: Match the activity below with the correct enzyme. (You won't use all the enzymes listed.) RNA acts…

A: DNA helicase is used during the DNA replication process where it binds to the double-stranded DNA…

Q: When designing primers that will have an added restriction enzyme site, you should a. ensure that…

A: Introduction  A primer is a small sequence of nucleic acids that serves as the precursor to DNA…

Q: How would the results of this activity have been different if the DNA sequence you digested were…

A: Gel electrophoresis is a process in which DNA fragments are separated on the basis of their size.…

Q: Meselson and Stahl grew bacteria in medium containing heavy nitrogen. After many cell divisions, the…

A: From Meselson and Stahl's experiment we have known that DNA replication is semi-conservative.

Q: You desperately need to join two DNA fragments, one with an EcoRl site and the other with a BamHI…

A: Based on the information provided, the correct statement is:C. You can fill in the ends with DNA…

Q: you performed a sanger sequencing on a particular segment of DNA. Explain at the molecular level…

A: Effect on gel profile when we either forgot to add ddNTPs Or add more ddNTPs in test tubes during…

Q: Chromosomal DNA fragments for molecular cloning are cut by _________, and inserted into molecular…

A: Answer - Option B - Restriction endonucleases; Ligase

Q: With regard to dideoxy sequencing, which of the following statementsis false?a. The dideoxy…

A: Nucleotides are the molecules composed of a sugar moiety, phosphate group, and a nitrogenous base.…

Q: By mistake, you add two primers to your DNA sequencing reaction. The primers anneal on the same…

A: The correct answer is:B. The detector will detect two nucleotides at about 75% of the positions.

Q: Why does Northern blotting not require the use of restriction enzymes?

A: Restriction enzyme naturally cleaves DNA because it's have double stranded sequence of neucleiotides…

Q: Sanger sequencing (aka the chain termination) a. is a method for for determining the sequence of…

A: Without a doubt!Identifying the order of nucleotides in a section of DNA may be accomplished by the…

Q: Based on the restriction enzyme specificities given below, what was the enzyme utilized to produce…

A: The corner stone of genetic engineering or recombinant DNA technology is a special class of enzymes…

Q: Compatible ends generated by restriction enzyme can be connected by ligase. Determine which of the…

A: Restriction enzymes are proteins that cut DNA at specific sequences. These sequences are usually…

Q: You performed RADseq analysis of two genomes using the restriction enzyme EcoRI. From each genome,…

A: * Restriction site associated DNA (RAD) markers are genetic marker that are used for association…

Q: a. The reading frame DNA sequence is  b. The mRNA sequence is  c. The polypeptide sequence is…

A: A "nucleic acid" is a linear polymer of nucleotides that is a component of the cell's information…

Q: plasmid has a total size of 2686 bp. There is an EcoRI recognition site at position 439, BamHI…

A: Plasmid  It is an extra chromosomal piece of DNA in the cell.

Q: You are tasked with amplifying a gene of interest with PCR. a. After performing a Southern blot, you…

A: Polymerase Chain Reaction(PCR)  is a technique used to  amplify the number of DNA or RNA strands.…

Q: Certain restriction endonucleases produce cohesive (sticky) ends.  This means that they: a. stick…

A: The primary purpose of restriction endonucleases is to guard against foreign genetic material,…

Q: 1. A DNA sequence 10 kb long contains one site at which it may be cut by the restriction enzyme…

A: Restriction enzymeIt refers to the enzyme that recognizes specific DNA sequences and cleaves the DNA…

Q: Based on the restriction enzyme specificities given below, what was the enzyme utilized to produce…

A: A restriction enzyme, also known as a restriction endonuclease, is a bacterial protein that cleaves…

Q: If you are a genetic engineer and you cloned your gene of interest in a plasmid and you want to know…

A: Answer. Blotting describes the immobilization of sample nucleic acids/proteins onto a solid support.…

Q: has been assembled by researchers and transplanted into a donor bacterial strain to study never…

A: A- recombinant plasmid  B- developmental genetics

Q: As you learned in this chapter,restriction enzymes are sophisticated“scissors” that geneticistsand…

A: Making many, exact copies of a specific segment of DNA is known as DNA cloning. The gene or other…

Q: Is it possible that two different restriction enzymes couldcut the human genome into exactly the…

A: Restriction enzymes are the type of nucleases that cleaves the nucleic acid at the intrinsic sites.…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.